Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

• Published in: Cells (Basel, Switzerland) Vol. 9; no. 1; p. 88
• Main Authors: Cutarelli, Alessandro, Ghio, Simone, Zasso, Jacopo, Speccher, Alessandra, Scarduelli, Giorgina, Roccuzzo, Michela, Crivellari, Michele, Maria Pugno, Nicola, Casarosa, Simona, Boscardin, Maurizio, Conti, Luciano
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.12.2019; MDPI
• Subjects: 3d culture; Arrays; Astrocytes - cytology; Cell culture; Cell Culture Techniques - methods; Cell Differentiation - physiology; Cell growth; Cerebral cortex; Cerebral Cortex - cytology; Cytomegalovirus; Embryogenesis; Epidermal growth factor; Fibroblasts; Growth conditions; hipsc-derived neural progenitors; human cortical progenitors; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Neurons - cytology; Physiology; Pluripotency; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Progenitor cells; Radial glial cells; Scanning electron microscopy; Silicon; Silicon - chemistry; Silicon - metabolism; silicon pillars; Silicon wafers; Stem cells; Tissue engineering; Tissue Engineering - methods; Italy; cell growth; cerebral cortex; hiPSC-derived neural progenitors; 3D culture; silicon pillars; human cortical progenitors
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells9010088

Silicon is a promising material for tissue engineering since it allows to produce micropatterned scaffolding structures resembling biological tissues. Using specific fabrication methods, it is possible to build aligned 3D network-like structures. In the present study, we exploited vertically-aligned silicon micropillar arrays as culture systems for human iPSC-derived cortical progenitors. In particular, our aim was to mimic the radially-oriented cortical radial glia fibres that during embryonic development play key roles in controlling the expansion, radial migration and differentiation of cortical progenitors, which are, in turn, pivotal to the establishment of the correct multilayered cerebral cortex structure. Here we show that silicon vertical micropillar arrays efficiently promote expansion and stemness preservation of human cortical progenitors when compared to standard monolayer growth conditions. Furthermore, the vertically-oriented micropillars allow the radial migration distinctive of cortical progenitors in vivo. These results indicate that vertical silicon micropillar arrays can offer an optimal system for human cortical progenitors' growth and migration. Furthermore, similar structures present an attractive platform for cortical tissue engineering.