Peroxiredoxin I deficiency attenuates phagocytic capacity of macrophage in clearance of the red blood cells damaged by oxidative stress

• Published in: BMB reports Vol. 45; no. 10; pp. 560 - 564
• Main Authors: Han, Y.H., Aging Research Center, KRIBB, Daejeon, Republic of Korea, Kwon, T.H., Aging Research Center, KRIBB, Daejeon, Republic of Korea, Kim, S.U., Aging Research Center, KRIBB, Daejeon, Republic of Korea, Ha, H.L., Aging Research Center, KRIBB, Daejeon, Republic of Korea, Lee, T.H., Aging Research Center, KRIBB, Daejeon, Republic of Korea, Kim, J.M., Chungnam National University, Daejeon, Republic of Korea, Jo, E.K., Chungnam National University, Daejeon, Republic of Korea, Kim, B.Y., Korea Research Institute of Bioscience and Biotechnology, Cheongwon, Republic of Korea, Yoon, D.Y., Konkuk University, Seoul, Republic of Korea, Yu, D.Y., Aging Research Center, KRIBB, Daejeon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01.10.2012; 생화학분자생물학회
• Subjects: ANAEMIA; ANEMIA; Anemia, Hemolytic - metabolism; Anemia, Hemolytic - pathology; ANEMIE; Aniline Compounds - pharmacology; Animals; Erythrocytes - drug effects; Erythrocytes - metabolism; FAGOCITOSIS; HAEMOLYSIS; Heinz Bodies - metabolism; Heinz body; HEMOLISIS; HEMOLYSE; Hemolytic anemia; MACROFAGOS; MACROPHAGE; MACROPHAGES; Macrophages - immunology; Macrophages - physiology; Mice; Mice, Knockout; Oxidative Stress; Peroxiredoxin; Peroxiredoxins - deficiency; Peroxiredoxins - genetics; Peroxiredoxins - metabolism; PHAGOCYTOSE; PHAGOCYTOSIS; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/bmbrep.2012.45.10.082

The role of peroxiredoxin (Prx) I as an erythrocyte antioxidant defense in red blood cells (RBCs) is controversial. Here we investigated the function of Prx I by using Prx Ⅰ∨-/- and Prx Ⅰ/Ⅱ∨-/- mice. Prx Ⅰ∨-/- mice exhibited a normal blood profile. However, Prx Ⅰ∨-/- mice showed more significantly increased Heinz body formation as compared with Prx Ⅱ∨-/- mice. The clearance rate of Heinz body-containing RBCs in Prx I∨-/- mice decreased significantly through the treatment of aniline hydrochloride (AH) compared with wild-type mice. Prx I deficiency decreased the phagocytic capacity of macrophage in clearing Heinz body-containing RBCs. Our data demonstrate that Prx I deficiency did not cause hemolytic anemia, but showed that further increased hemolytic anemia symptoms in Prx Ⅱ∨-/- mice by attenuating phagocytic capacity of macrophage in oxidative stress damaged RBCs, suggesting a novel role of Pi, I in phagocytosis of macrophage.