Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity

• Published in: Microbiology (Society for General Microbiology) Vol. 147; no. 11; pp. 2885 - 2895
• Main Authors: King, Audrey J, Berbers, Guy, van Oirschot, Hans F. L. M, Hoogerhout, Peter, Knipping, Karen, Mooi, Frits R
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2001; Society for General Microbiology
• Subjects: Amino Acid Sequence; Animals; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - immunology; Antibodies, Monoclonal - immunology; Bacterial diseases; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Bacterial Vaccines - immunology; Bacteriology; Base Sequence; Biological and medical sciences; Bordetella pertussis; Bordetella pertussis - genetics; Bordetella pertussis - immunology; Bordetella pertussis - pathogenicity; Cross Reactions; Disease Models, Animal; DNA, Bacterial; Epitope Mapping; Epitopes, B-Lymphocyte - immunology; Experimental bacterial diseases and models; Fundamental and applied biological sciences. Psychology; Humans; Infectious diseases; Medical sciences; Mice; Microbiology; Molecular Sequence Data; pertactin; Polymorphism, Genetic; Protein Structure, Tertiary; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Virulence Factors, Bordetella; Whooping Cough - immunology; Divergence; Respiratory disease; Countries; Vaccine strain; Vaccination; Rodentia; Vaccine; Bordetella pertussis; Adhesion; Immunity; Protein; Whooping cough; Infection; Relevance; Vertebrata; Mammalia; Antigenicity; Mouse; Vaccination coverage; Emergence; Bacteriosis; Bacteria; Polymorphism
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-147-11-2885

Research Laboratory for Infectious Diseases 1 , Laboratory for Clinical Vaccine Research 2 and Laboratory for Vaccine Research 3 , National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands Eijkman Winkler Institute, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands 4 Author for correspondence: Frits R. Mooi. Tel: +31 30 2743091. Fax: +31 30 2744449. e-mail: fr.mooi{at}rivm.nl In several countries pertussis is re-emerging, despite a high vaccination coverage. It is suggested that antigenic divergence between Bordetella pertussis vaccine strains and circulating strains, in particular with respect to pertactin, has contributed to pertussis re-emergence. Polymorphism in pertactin is essentially limited to region 1, which is composed of repeats and is located adjacent to an Arg-Gly-Asp motif implicated in adherence. Evidence is provided for the immunological relevance of polymorphism in region 1. Region 1 was found to contain a B-cell epitope recognized in both humans and mice. Furthermore, variation in region 1 affected antibody binding and, in a mouse respiratory infection model, the efficacy of a whole-cell vaccine. Moreover, passive and active immunization indicated that region 1 confers protective immunity. An mAb directed against a linear conserved epitope conferred cross-immunity against isolates with distinct pertactin variants. The results indicate an important role of region 1 of pertactin in immunity. Keywords: antigenic variation, P.69/pertactin, immunization, protective immunity Abbreviations: HRP, horseradish peroxidase; MBP, maltose-binding protein; WCV, whole-cell pertussis vaccine The GenBank accession numbers for the sequences reported in this paper are AJ011015 , AJ011016 , AJ011091 , AJ011092 , AJ011093 , AJ132095 , AJ245927 and X54547 .