In Vitro Selection of N-Peptide-Binding RNA on a Quartz-Crystal Microbalance to Study a Sequence-Specific Interaction between the Peptide and Loop RNA

• Published in: Chembiochem : a European journal of chemical biology Vol. 4; no. 2-3; pp. 217 - 220
• Main Authors: Furusawa, Hiroyuki, Murakawa, Akiko, Fukusho, Shinobu, Okahata, Yoshio
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 03.03.2003; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Amino Acid Sequence; Base Sequence; Binding Sites; Molecular Sequence Data; Nucleic Acid Conformation; peptides; Peptides - chemistry; Peptides - metabolism; Quartz; quartz-crystal microbalance; RNA; RNA - chemistry; RNA - metabolism; RNA recognition; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - metabolism; selection
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.200390034

On‐the‐spot RNA selection: N peptide or mutated N peptide was immobilized on a highly sensitive 27‐MHz quartz‐crystal microbalance, which was used as a monitoring and evaluation device for in vitro selection of RNA sequences that bind to these peptides (see figure). This method allowed the study of a sequence‐specific interaction of the peptide with loop RNA in situ and without the use of labeling techniques and time‐consuming separate binding assays.