Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Anion-Exchange Chromatography of Bovine Serum Albumin

• Published in: Biotechnology progress Vol. 15; no. 4; pp. 740 - 752
• Main Authors: Miyabe, Kanji, Guiochon, Georges
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 01.07.1999; American Institute of Chemical Engineers
• Subjects: Adsorption; Animals; Anions; Biological and medical sciences; Biotechnology; Cattle; Cells; Chemical Phenomena; Chemistry, Physical; Chromatography, Ion Exchange - methods; Diffusion; Dispersions; Fundamental and applied biological sciences. Psychology; Ion exchange resins; Kinetics; Mass transfer; Mathematical models; Methods. Procedures. Technologies; Models, Chemical; Nonlinear Dynamics; Others; Phase equilibria; Porosity; Reaction kinetics; Serum Albumin, Bovine - chemistry; Surface Properties; Thermodynamic properties; Various methods and equipments; Bovine; Albumin; Protein; Mass transfer; Ion exchange chromatography; Anion exchange; Kinetic model; Separation method; Vertebrata; Mammalia; Mass transfer coefficient; Adsorption; Concentration effect; Artiodactyla; Kinetics; Ungulata
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp990062x

The experimental results of a previous study of the mass transfer kinetics of bovine serum albumin (BSA) in ion‐exchange chromatography under nonlinear conditions are reevaluated. The analysis of the concentration dependence of the lumped mass‐transfer rate coefficient (km,L) provides information on the kinetics of axial dispersion, fluid‐to‐particle mass transfer, intraparticle mass transfer, and adsorption/desorption. The new analysis shows that the contribution of intraparticle mass transfer is the dominant one. Similar to km,L, the surface diffusivity (Ds) of BSA increases with increasing concentration. The linear concentration dependence of km,L seems to originate in a similar dependence of Ds. The use of an heterogeneous‐surface model for the anion‐exchange resin provides an explanation of the positive concentration dependence of Ds. This work illustrates how frontal analysis data can be used for a detailed investigation of the kinetics of mass transfer between the phases of a chromatographic column, in addition to its conventional use in the determination of the thermodynamic characteristics of the phase equilibrium.