Ca2+-Sensitization of Contraction in the h1 Calponin-Deficient Smooth Muscle

• Published in: Japanese journal of pharmacology Vol. 84; no. 4; pp. 474 - 475
• Main Authors: Yoshimoto, Ryo, Hori, Masatoshi, Takahashi, Katsuhito, Taniguchi, Shun-ichiro, Katsuki, Motoya, Ozaki, Hiroshi, Karaki, Hideaki
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Pharmacological Society 2000
• Subjects: Animals; Aorta - drug effects; Aorta - physiology; Ca2+-sensitization; Calcium Chloride - metabolism; Calcium Chloride - pharmacology; Calcium-Binding Proteins - deficiency; Calcium-Binding Proteins - physiology; Calponin; Calponins; Dinoprost - pharmacology; Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology; Ileum; Mice; Microfilament Proteins; Muscle Contraction - drug effects; Muscle Contraction - physiology; Muscle, Smooth - drug effects; Muscle, Smooth - physiology; Oxytocics - pharmacology; Phorbol Esters - pharmacology; Smooth muscle contraction; Smooth muscle contraction; Ca2+-sensitization; Calponin
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1254/jjp.84.474

Role of hi calponin on Ca2+-sensitivity of smooth muscle contraction was investigated using h1 calponin gene-deficient mice (CP−/−) and wild type mice (CP+/+). PGF2α induced a comparable force in intact aorta of CP+/+ and CP−/−. DPB showed similar effects to PGF2α. In membrane-permeabilized ileal smooth muscle, PDBu enhanced Ca2+-sensitivity of contraction comparably in CP+/+ and CP−/−. GTPγ-S showed similar effects. Our results suggest that hi calponin does not regulate Ca2+-sensitivity in the contractile mechanism of smooth muscle.