Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss

Recurrent pregnancy loss (RPL) is a major reproductive problem that affects approximately 5% of couples. The objective of this study was to assess vaginal flora dysbiosis in women suffering from unexplained RPL and to investigate the pathogenic properties of the microbiota associated with aerobic va...

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Published inDiagnostics (Basel) Vol. 12; no. 10; p. 2444
Main Authors Ncib, Karima, Bahia, Wael, Leban, Nadia, Mahdhi, Abdelkarim, Trifa, Fatma, Mzoughi, Ridha, Haddad, Anis, Jabeur, Chédia, Donders, Gilbert
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.10.2022
MDPI
Subjects
Abortion, Habitual
aerobic vaginitis
antibiotic resistance
Antibiotics
Bacteria
Bacterial infections
biofilm formation
Biofilms
Causes of
Development and progression
dysbiosis
Health aspects
Infections
lactobacillus
Metabolism
Microbiota (Symbiotic organisms)
Microorganisms
Microscopy
Miscarriage
Pathogens
Pregnancy
recurrent pregnancy loss
Risk factors
Vagina
Vaginitis
Womens health
Tunisia
United Kingdom > UK
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Summary:Recurrent pregnancy loss (RPL) is a major reproductive problem that affects approximately 5% of couples. The objective of this study was to assess vaginal flora dysbiosis in women suffering from unexplained RPL and to investigate the pathogenic properties of the microbiota associated with aerobic vaginitis (AV). The study included one hundred fifteen women, 65 with RPL and 50 controls. The diversity of vaginal microbiota isolated was evaluated by molecular sequencing. Then, pathogenic factors, such as acid-resistance, antibiotics susceptibility, and biofilm formation were evaluated. The prevalence of AV was five-fold higher in the RPL group than in the controls (64.6% vs. 12.0%). The most prevalent isolates in the case group were Enterococcus spp. (52%) and Staphylococcus spp. (26%). All bacterial strains tolerate low pH. The prevalence of multidrug resistance (MDR) among all bacteria was 47.7%. Of all strains, 91.0% were biofilm producers. The presence of MDR was found to be related to biofilm formation. The results provide evidence supporting an increased presence of dysbiosis of the vaginal flora, especially AV, in women with RPL in Tunisia. The viability of the AV-associated bacteria and their persistence in the genitals may be due to their ability to resist low pH and to produce a biofilm.
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics12102444