Using proximity extension proteomics assay to identify biomarkers associated with infarct size and ejection fraction after ST-elevation myocardial infarction

• Published in: Scientific reports Vol. 10; no. 1; p. 18663
• Main Authors: Mohammad, Moman A., Koul, Sasha, Egerstedt, Anna, Smith, J. Gustav, Noc, Marko, Lang, Irene, Holzer, Michael, Clemmensen, Peter, Gidlöf, Olof, Metzler, Bernhard, Engstrøm, Thomas, Erlinge, David
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.10.2020; Nature Publishing Group
• Subjects: 692/4019/2776; 692/4019/592; 692/53/2422; 692/53/2423; Aged; Apoptosis; Biomarkers - metabolism; Cardiac and Cardiovascular Systems; Clinical Medicine; Cystatins; Cytokines; Ejection fraction; Female; Heart; Heart attacks; Humanities and Social Sciences; Humans; Inflammation; Interleukin 10; Interleukin 16; Interleukin 6; Kardiologi; Klinisk medicin; Magnetic resonance imaging; Male; Medical and Health Sciences; Medicin och hälsovetenskap; Middle Aged; multidisciplinary; Myocardial infarction; Myoglobins; Pentraxins; Proteins; Proteomics; Proteomics - methods; Renin; Science; Science (multidisciplinary); ST Elevation Myocardial Infarction - diagnostic imaging; ST Elevation Myocardial Infarction - metabolism; ST Elevation Myocardial Infarction - physiopathology; Tumor necrosis factor; Ventricle
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-75399-6

Plasma concentrations of many cardiovascular and inflammatory proteins are altered after ST-elevation myocardial infarction (STEMI) and may provide prognostic information. We conducted a large-scale proteomic analysis in patients with STEMI, correlating protein levels to infarct size and left ventricular ejection fraction (LVEF) determined with cardiac magnetic resonance imaging. We analysed 131 cardiovascular and inflammatory proteins using a multiplex proximity extension assay and blood samples obtained at baseline, 6, 24, and 96 h from the randomised clinical trial CHILL-MI. Cardiac magnetic resonance imaging data at 4 ± 2 days and 6 months were available as per trial protocol. Using a linear regression model with bootstrap resampling and false discovery rate adjustment we identified five proteins (ST2, interleukin-6, pentraxin-3, interleukin-10, renin, and myoglobin) with elevated values corresponding to larger infarct size or worse LVEF and four proteins (TNF-related apoptosis-inducing ligand, TNF-related activation induced cytokine, interleukin-16, and cystatin B) with values inversely related to LVEF and infarct size, concluding that among 131 circulating inflammatory and cardiovascular proteins in the acute and sub-acute phase of STEMI, nine showed a relationship with infarct size and LVEF post-STEMI, with IL-6 and ST2 exhibiting the strongest association.