A combination of rebaudioside A and neohesperidin dihydrochalcone suppressed weight gain by regulating visceral fat and hepatic lipid metabolism in ob/ob mice

• Published in: Food science and biotechnology Vol. 33; no. 4; pp. 913 - 923
• Main Authors: Kim, Yeri, Han, Hyejin, Oh, Yeonsoo, Shin, Hakdong, Park, Gwoncheol, Park, Sunghee, Manthey, John A., Kim, Yang, Kim, Yuri
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.03.2024; Springer Nature B.V; 한국식품과학회
• Subjects: Adipogenesis; alanine transaminase; Animal models; Body fat; Body weight; Body weight gain; Chemistry; Chemistry and Materials Science; Fat metabolism; Food Science; Genes; Glutamic oxaloacetic transaminase; Inflammation; Intestinal microflora; intestinal microorganisms; Lipid metabolism; Lipids; Lipogenesis; Lipolysis; Liver; Metabolic disorders; Mucispirillum; neohesperidin; Neohesperidin dihydrochalcone; Nutrition; Obesity; Oxidation; Research Article; Sweeteners; Transaminase; triacylglycerols; Triglycerides; visceral fat; weight gain; 식품과학; Obesity; Metabolic diseases; Microbiota; Rebaudioside A; Neohesperidin dihydrochalcone
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-023-01391-1

Rebaudioside A (Reb A) and neohesperidin dihydrochalcone (NHDC) are known as intense sweeteners. This study aimed to examine the anti-obesity effects of Reb A and NHDC. C57BL/6 J- ob/ob mice were supplemented with Reb A (50 mg/kg body weight [b.w.]), NHDC (100 mg/kg b.w.), or their combination (COMB) for 4 weeks. COMB-supplemented mice showed significant reduction in b.w. gain, food efficiency ratio, and fat mass. Additionally, mice in the COMB group showed suppressed levels of genes related to adipogenesis, lipogenesis, and lipolysis in the perirenal fat and the levels of hepatic triglyceride, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase. The lipogenesis and pro-inflammatory gene expressions were also downregulated in the liver, whereas β -oxidation related genes were upregulated in the COMB group. In addition, mice that received COMB showed distinct gut microbiota structure, enriched in Blautia and Parabacteroides , and depleted in Faecalibaculum and Mucispirillum , in relation to the control group. These results suggest that supplementation with Reb A and NHDC may be an effective treatment for obesity-related metabolic disorders.