Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy

The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated sta...

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Bibliographic Details
Published inJournal of biomedical optics Vol. 19; no. 4; p. 046022
Main Authors Doblas, Ana, Sánchez-Ortiga, Emilio, Martínez-Corral, Manuel, Saavedra, Genaro, Garcia-Sucerquia, Jorge
Format Journal Article
LanguageEnglish
Published United States Society of Photo-Optical Instrumentation Engineers 01.04.2014
Subjects
Animals
Biological
Digital
Drosophila melanogaster
Erythrocytes - cytology
Head - anatomy & histology
Holography - methods
Image Processing, Computer-Assisted - methods
Imaging
Microscopes
Microscopy
Microscopy - methods
Red blood cells
Stability
digital holographic microscopy
aberration compensation
quantitative phase imaging
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Summary:The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.
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ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.19.4.046022