Exopolysaccharide biosynthesis in Lactococcus lactis NIZO B40: functional analysis of the glycosyltransferase genes involved in synthesis of the polysaccharide backbone
We used homologous and heterologous expression of the glycosyltransferase genes of the Lactococcus lactis NIZO B40 eps gene cluster to determine the activity and substrate specificities of the encoded enzymes and established the order of assembly of the trisaccharide backbone of the exopolysaccharid...
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Published in | Journal of Bacteriology Vol. 181; no. 1; pp. 338 - 340 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Microbiology
1999
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Series | Note |
Subjects | |
Online Access | Get full text |
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Summary: | We used homologous and heterologous expression of the glycosyltransferase genes of the Lactococcus lactis NIZO B40 eps gene cluster to determine the activity and substrate specificities of the encoded enzymes and established the order of assembly of the trisaccharide backbone of the exopolysaccharide repeating unit. EpsD links glucose-1-phosphate from UDP-glucose to a lipid carrier, EpsE and EpsF link glucose from UDP-glucose to lipid-linked glucose, and EpsG links galactose from UDP-galactose to lipid-linked cellobiose. Furthermore, EpsJ appeared to be involved in EPS biosynthesis as a galactosyl phosphotransferase or an enzyme which releases the backbone oligosaccharide from the lipid carrier. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Microbial Ingredients Section, NIZO Food Research, Kernhemseweg 2, 6718 ZB, Ede, The Netherlands. Phone: 31 318 659511. Fax: 31 318 650400. E-mail: kranenbu@nizo.nl. Present address: Laboratory of Microbiology, Centre for Neurosciences of Coimbra, University of Coimbra, 3049 Coimbra Codex, Portugal. |
ISSN: | 0021-9193 1098-5530 1067-8832 |
DOI: | 10.1128/jb.181.1.338-340.1999 |