Comparison of the AF146527 and B1 Repeated Elements, Two Real-Time PCR Targets Used for Detection of Toxoplasma gondii

Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that th...

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Published inJournal of Clinical Microbiology Vol. 48; no. 2; pp. 591 - 592
Main Authors Wahab, Tara, Edvinsson, Benjamin, Palm, Daniel, Lindh, Johan
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.02.2010
American Society for Microbiology (ASM)
Subjects
Animals
Biological and medical sciences
Clinical Laboratory Techniques - methods
DNA, Protozoan - genetics
Fundamental and applied biological sciences. Psychology
Humans
Medicin och hälsovetenskap
Microbiology
Molecular Diagnostic Techniques - methods
Parasitology
Polymerase Chain Reaction - methods
Repetitive Sequences, Nucleic Acid
Sensitivity and Specificity
Toxoplasma - genetics
Toxoplasma - isolation & purification
Toxoplasma gondii
Toxoplasmosis - diagnosis
Toxoplasmosis - parasitology
Polymerase chain reaction
Protozoa
Apicomplexa
Toxoplasma gondii
Detection
Real time
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Summary:Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that the B1 gene may be the preferred diagnostic target.
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Present address: European Centre for Disease Prevention and Control, 171 83 Stockholm, Sweden.
ISSN:0095-1137
1098-660X
1098-660X
DOI:10.1128/JCM.01113-09