Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation
To achieve high time–space efficiency for sophorolipid production with yeast Candida bombicola , a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal...
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Published in | Applied microbiology and biotechnology Vol. 97; no. 3; pp. 1103 - 1111 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer-Verlag
01.02.2013
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | To achieve high time–space efficiency for sophorolipid production with yeast
Candida bombicola
, a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80 g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200 g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24 h), highlighting the industrial potential of the platform developed in this work. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0175-7598 1432-0614 |
DOI: | 10.1007/s00253-012-4399-z |