Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation

To achieve high time–space efficiency for sophorolipid production with yeast Candida bombicola , a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal...

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Published inApplied microbiology and biotechnology Vol. 97; no. 3; pp. 1103 - 1111
Main Authors Gao, Renjun, Falkeborg, Mia, Xu, Xuebing, Guo, Zheng
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.02.2013
Springer
Springer Nature B.V
Subjects
Biomass
Biomedical and Life Sciences
Biotechnological Products and Process Engineering
Biotechnology
Biotechnology - methods
Candida
Candida - growth & development
Candida - metabolism
Candida bombicola
Carbon sources
Cell Count
Cell growth
Culture Media - chemistry
Fermentation
Glucose
Glycerol
Glycolipids
Life Sciences
Lipid Metabolism
Microbial Genetics and Genomics
Microbiological research
Microbiology
Nutrient concentrations
Physiological aspects
Productivity
Studies
Surface active agents
Surfactants
Time Factors
Yeast
Yeasts
Biosurfactants
Volumetric productivity
High cell density fermentation
Sophorolipids
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Summary:To achieve high time–space efficiency for sophorolipid production with yeast Candida bombicola , a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80 g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200 g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24 h), highlighting the industrial potential of the platform developed in this work.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-012-4399-z