Relationship Between Oxygen Sensitivity and Oxygen Metabolism of Bifidobacterium Species

• Published in: Journal of dairy science Vol. 75; no. 12; pp. 3296 - 3306
• Main Authors: Shimamura, Seiichi, Abe, Fumiaki, Ishibashi, Norio, Miyakawa, Hiroshi, Yaeshima, Tomoko, Araya, Tomoko, Tomita, Mamoru
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.12.1992; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: Bifidobacterium; Bifidobacterium - drug effects; Bifidobacterium - growth & development; Bifidobacterium - metabolism; Bifidobacterium adolescentis; Bifidobacterium breve; Bifidobacterium infantis; Bifidobacterium longum; Biological and medical sciences; Food industries; Fundamental and applied biological sciences. Psychology; Hydrogen Peroxide - metabolism; Hydrogen-Ion Concentration; Milk and cheese industries. Ice creams; Multienzyme Complexes - metabolism; NADH, NADPH Oxidoreductases - metabolism; nicotinamide adenine dinucleotide-oxidase; nicotinamide adenine dinucleotide-peroxidase; Oxygen - metabolism; Oxygen - pharmacology; oxygen sensitivity; Peroxidases - metabolism; Superoxide Dismutase - metabolism; SOD; Bifidobacterium; NADH; nicotinamide adenine dinucleotide-oxidase; nicotinamide adenine dinucleotide-peroxidase; oxygen sensitivity; Cell proliferation; Oxygen; Actinomycetaceae; NAD+ peroxidase; Enzyme; Superoxide dismutase; Hydrogen Peroxides; Actinomycetales; Enzymatic activity; pH; Bacteria; Actinomycetes
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(92)78105-3

Bifidobacteria, which are obligate anaerobes, were studied to determine the relationship between their sensitivity to oxygen and oxygen metabolism. Among the four species tested, Bifidobacterium infantis, Bifidobacterium breve, and Bifidobacterium longum differed from Bifidobacterium adolescentis in sensitivity to oxygen. The former three species showed marked growth under conditions of partial aeration, whereas the growth of B. adolescentis was suppressed by low concentrations of oxygen. Bifidobacteria express reduced NAD-oxidase and -peroxidase activities, which function in a pathway for two-electron reduction of molecular oxygen, producing hydrogen peroxide and, subsequently, water. Activities of reduced NAD-oxidase and -peroxidase were inversely correlated with their sensitivities to oxygen. Bifidobacterium adolescentis exhibited lowered activities of these two enzymes; the activities were 10 to 20% of those observed with B. infantis, B. breve, and B. longum. These observations are compatible with the hypothesis that reduced NAD-oxidase and reduced NAD-peroxidase in Bifidobacterium species play a role in prevention of oxygen toxicity. Superoxide dismutase activity was also detected in Bifidobacterium species. Superoxide dismutase is probably not involved in detoxification of oxygen, because the activity of this enzyme was extremely low, and the sensitivity to oxygen varied independently of superoxide dismutase activity.