Development and Application of a New Method for Assay of the Content of Hoelen Extract in Chinese Traditional Medicines

• Published in: Chemical & pharmaceutical bulletin Vol. 38; no. 11; pp. 3064 - 3068
• Main Authors: TANIMORI, Hideaki, MAEDA, Takako, AKAHORI, Akira, SONOI, Shinsuke, KONDO, Seizo, KITAGAWA, Tsunehiro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 25.11.1990; Maruzen; Japan Science and Technology Agency
• Subjects: Biological and medical sciences; Chinese traditional medicine; extract content; General pharmacology; hoelen; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; SAEIA; selected antibody enzyme immunoassay; Basidiomycetes; Extract; Enzyme immunoassay; Immunological method; Medicinal plant; Fungi; Poria; Folk medicine; Investigation method; Asia; China; Plant origin; Qualitative analysis; Thallophyta
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.38.3064

A newly selected antibody enzyme immunoassay (SAEIA) for aqueous hoelen extract was developed. Hoelen fragments were immunized and an antiserum specific for hoelen was elicited in rabbits. An aqueous extract of hoelen was converted to a solid-phase antigen using microtiter plates. The efficiency for immobilization of hoelen extract to three types of microtiter plates was compared and carbo-plate was chosen as the solid-phase antigen support. A new assay method for a hoelen extract, with a working range between 3 ng and 30 μg, was developed applying the novel assay principle of SAEIA. The selective binding of the antibodies specific for hoelen extract, contained in the anti-hoelen serum, to the solid-phase antigen, was detected using β-D-galactosidase labeled anti-rabbit immunoglobulin G as a tracer. Specificity of the SAEIA to the hoelen extract was also demonstrated. The contents of hoelen extract in three Chinese traditional medicines were successfully analyzed applying the SAEIA method.