Heat shock-induced three-dimensional-like proliferation of normal human fibroblasts mediated by pressed silk

• Published in: International journal of molecular sciences Vol. 10; no. 11; pp. 4963 - 4976
• Main Authors: Hiragami, Fukumi, Motoda, Hirotoshi, Takezawa, Toshiaki, Takabayashi, Chiyuki, Inoue, Shigeki, Wakatake, Yuji, Kano, Yoshio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.11.2009; Molecular Diversity Preservation International (MDPI)
• Subjects: Apoptosis; Cell Culture Techniques; Cell growth; Cell Line; Cell Proliferation - drug effects; Cell Survival - drug effects; Experiments; Fever; Fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Heat treating; heat treatment; Hot Temperature; Hsp27; HSP27 Heat-Shock Proteins - metabolism; Humans; Hyperthermia; Kinases; Male; normal human dermal fibroblasts; p38 MAPK; p38 Mitogen-Activated Protein Kinases - metabolism; Phosphorylation - drug effects; Physical therapy; Pressure ulcers; Silk - chemistry; Silk - pharmacology; Temperature; Thermotherapy; three-dimensional-like proliferation patterns; Wound healing; Japan; three-dimensional-like proliferation patterns; heat treatment; p38 MAPK; Hsp27; normal human dermal fibroblasts
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms10114963

The aim of this study was to determine the optimal heat treatment conditions for enhancement of pressed silk-mediated 3D-like proliferation of normal human dermal fibroblasts, as well as to determine the responses to heat shock of cells and intracellular signaling pathways. The beginning of 3D-like pattern formation of cells was observed in the second week after the start of the experiment. The mean rates of beginning of 3D-like pattern formation by cells heat-treated at 40 masculineC and 43 masculineC for 10 min were significantly higher (3.2- and 8.6-fold, respectively) than that of untreated cells. We found that apoptosis had occurred in 7.5% and 50.0% of the cells at one week after heat treatment for 10 min at 43 masculineC and 45 masculineC, respectively. Western blot analysis demonstrated that phosphorylation of p38 MAPK and that of Hsp27 were markedly increased by heat treatment at 43 masculineC for 10 min. The results of an experiment using a p38 MAPK inhibitor and Hsp27 inhibitor suggest that activation of p38 MAPK by heat shock is associated with 3D-like cell proliferation and that Hsp27 contributes to the inhibition of apoptosis. The results of this study should be useful for further studies aimed at elucidation of the physiologic mechanisms underlying thermotherapy.