Enhanced Osteogenesis Potential of MG-63 Cells through Sustained Delivery of VEGF via Liposomal Hydrogel

• Published in: Gels Vol. 9; no. 7; p. 562
• Main Authors: Tsai, Milton Hongli, Megat Abdul Wahab, Rohaya, Zainal Ariffin, Shahrul Hisham, Azmi, Fazren, Yazid, Farinawati
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.07.2023; MDPI
• Subjects: Alizarin; Angiogenesis; Biocompatibility; Cell differentiation; Chemical properties; Comparative analysis; Differentiation; Health aspects; hydrogel; Hydrogels; Liposomes; MG-63 osteoblast-like cells; Mineralization; Morphology; osteogenesis; Pore size; Regeneration (physiology); Scaffolds; Scientific equipment and supplies industry; Skin & tissue grafts; Spectrum analysis; Vascular endothelial growth factor; Malaysia; United States; Massachusetts; Germany; MG-63 osteoblast-like cells; osteogenesis; hydrogel; liposomes; vascular endothelial growth factor
• ISSN: 2310-2861; 2310-2861
• DOI: 10.3390/gels9070562

The challenges of using VEGF to promote osteoblastic differentiation include a short half-life and a narrow therapeutic window. A carrier system combining hydrogel and liposomes may improve the therapeutic efficacy of VEGF for bone regeneration. This study aimed to investigate the effects of delivery of VEGF via liposomal hydrogel on the osteogenesis of MG-63 cells. Liposomal hydrogel scaffold was fabricated and then characterized in terms of the morphological and chemical properties using FESEM and FTIR. In 2.5D analysis, the MG-63 cells were cultured on liposomal hydrogel + VEGF as the test group. The osteogenic effects of VEGF were compared with the control groups, i.e., hydrogel without liposomes + VEGF, osteogenic medium (OM) supplemented with a bolus of VEGF, and OM without VEGF. Cell morphology, viability, and differentiation and mineralization potential were investigated using FESEM, MTT assay, ALP activity, and Alizarin red staining. The characterization of scaffold showed no significant differences in the morphological and chemical properties between hydrogel with and without liposomes ( > 0.05). The final 2.5D culture demonstrated that cell proliferation, differentiation, and mineralization were significantly enhanced in the liposomal hydrogel + VEGF group compared with the control groups ( < 0.05). In conclusion, liposomal hydrogel can be used to deliver VEGF in a sustained manner in order to enhance the osteogenesis of MG-63 cells.