Constructing an amylolytic brewing yeast Saccharomyces pastorianus suitable for accelerated brewing

An amylolytic brewing yeast Saccharomyces pastorianus, free of vector sequences and drug-resistance genes, was constructed by disrupting the α-acetolactate synthase gene and introducing the α-amylase gene as a selective marker. The resulting recombinant strain was able to utilize starch as the sole...

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Published inJournal of bioscience and bioengineering Vol. 98; no. 6; pp. 414 - 419
Main Authors Liu, Zengran, Zhang, Guangyi, Liu, Shigui
Format Journal Article
LanguageEnglish
Published Amsterdarm Elsevier B.V 2004
Elsevier Science
Elsevier Limited
Subjects
amylase
Biological and medical sciences
Biotechnology
brewing yeast
diacetyl
fermentation
Fundamental and applied biological sciences. Psychology
gene disruption
Saccharomyces pastorianus
fermentation
brewing yeast
amylase
diacetyl
gene disruption
Amylase
Enzyme
Brewing
Saccharomyces
Fermentation
Biacetyl
Fungi
Gene
Ascomycetes
Brewer yeast
Thallophyta
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Summary:An amylolytic brewing yeast Saccharomyces pastorianus, free of vector sequences and drug-resistance genes, was constructed by disrupting the α-acetolactate synthase gene and introducing the α-amylase gene as a selective marker. The resulting recombinant strain was able to utilize starch as the sole carbon source and its α-acetolactate synthase activity was lowered by 30%. Fermentation tests confirmed that the diacetyl concentration and the residual oligosaccharide were reduced by 70% and 25%, respectively, in fermented wort by the recombinant strain, while the brewing performance of the recombinant strain was retained.
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ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(05)00305-1