Complete genomic characterization and antigenic relatedness of genogroup III, genotype 2 bovine noroviruses

• Published in: Archives of virology Vol. 152; no. 2; pp. 257 - 272
• Main Authors: Oliver, S. L, Asobayire, E, Charpilienne, A, Cohen, J, Bridger, J. C
• Format: Journal Article
• Language: English
• Published: Wien Vienna : Springer-Verlag 01.02.2007; New York, NY Springer; Springer Nature B.V; Springer Verlag; Springer-Verlag
• Subjects: 5' Untranslated Regions; Amino Acid Motifs; Amino Acid Sequence; Amino acids; Animals; Antibodies; Antibodies, Viral - blood; Antigens, Viral - chemistry; Antigens, Viral - genetics; Antigens, Viral - immunology; Baculoviridae - genetics; Base Sequence; Biological and medical sciences; Capsid Proteins - chemistry; Capsid Proteins - genetics; Cattle; Cattle Diseases - virology; Cloning; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Genome, Viral; Genomes; Genotype; Genotype & phenotype; Life Sciences; Microbiology; Microbiology and Parasitology; Miscellaneous; Models, Molecular; Molecular Sequence Data; Molecular Weight; Norovirus; Norovirus - classification; Norovirus - genetics; Norovirus - immunology; Norovirus - isolation & purification; Open Reading Frames; Phylogeny; Polymerase Chain Reaction; Protein Structure, Tertiary; Proteins; RNA, Viral - genetics; RNA, Viral - isolation & purification; Rotavirus; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Serology; Serotyping; Species Specificity; Specific Pathogen-Free Organisms; Virion - genetics; Virion - immunology; Virion - isolation & purification; Virion - ultrastructure; Virology; Viruses; United Kingdom > UK; Vertebrata; Mammalia; Bovine; Genomics; Genotype; Artiodactyla; Veterinary; Ungulata; MOLECULAR CHARACTERIZATION; ENTERIC CALICIVIRUSES; PHYLOGENETIC ANALYSIS; GENOMIQUE; YOUNG CALVES; SEQUENCE; NORWALK-LIKE VIRUSES; EPIDEMIC VIRAL GASTROENTERITIS; EXPRESSION; EXPERIMENTAL-INFECTION
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-006-0856-2

Bovine enteric noroviruses form a genogroup, III, distinct from the 2 human norovirus genogroups, I and II. Two genogroup III genotypes were suggested by partial genomic analyses. In the present study, analysis of the full-length genome sequence of Bo/Newbury2/76/UK and the more contemporary Newbury2-like virus, Bo/Dumfries/1994/UK, showed that both were 7311 nucleotides in length and had three open reading frames (ORFs), amino acids motifs typical of noroviruses, and 95% or greater amino acid identities to each other in all regions of their genome. Apart from the ORF1 NTPase region, their ORF1 regions had less than 90% identity to the genogroup III genotype 1 Bo/Jena/80/DE virus, confirming two genogroup III genotypes. A close antigenic relationship was demonstrated by ELISA between the genotype 2 viruses, which will allow their serological diagnosis.