Elicitors’ influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions

• Published in: Applied microbiology and biotechnology Vol. 100; no. 11; pp. 4909 - 4922
• Main Authors: Biswas, Tanya, Kalra, Alok, Mathur, A. K., Lal, R. K., Singh, Manju, Mathur, Archana
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2016; Springer; Springer Nature B.V
• Subjects: Analysis; Bacillus; Bacillus - chemistry; Bacteria; Biomedical and Life Sciences; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; Cell interactions; Cellular biology; Cobalt; Cobalt - chemistry; Councils; Culture Media; Gene expression; Ginsenosides - biosynthesis; Hydrogen peroxide; Hydrogen Peroxide - chemistry; Industrial research; Korean ginseng; Life Sciences; Metabolism; Metabolites; Microbial Genetics and Genomics; Microbiology; Nickel - chemistry; Nitroprusside - chemistry; Observations; Panax - chemistry; Panax - cytology; Panax quinquefolius; Physiological aspects; Plant Cells - drug effects; Plant Cells - metabolism; Plant extracts; Productivity; Properties; Pseudomonas; Pseudomonas - chemistry; Sodium; Studies; Trichoderma - chemistry; Elicitation; Exudation; Cell suspension; Ginsenoside; Panax quinquefolius
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-015-7264-z

Cobalt nitrate, nickel sulphate, hydrogen peroxide, sodium nitroprusside, and culture filtrates of Pseudomonas monteili , Bacillus circularans , Trichoderma atroviridae , and Trichoderma harzianum were tested to elicit ginsenoside production in a cell suspension line of Panax quinquefolius . Abiotic elicitors preferentially increased panaxadiols whereas biotic elicitors upregulated the panaxatriol synthesis. Cobalt nitrate (50 μM) increased total ginsenosides content by twofold (54.3 mg/L) within 5 days. It also induced the Rc synthesis that was absent in the control cultures. Elicitation with P. monteili (2.5 %  v / v , 5 days) also supported 2.4-fold enhancement in saponin yield. Elicitation by T. atroviridae or hydrogen peroxide induced the synthesis of Rg3 and Rh2 that are absent in ginseng roots. The highest ginsenosides productivity (3.2-fold of control) was noticed in cells exposed to 1.25 %  v / v dose of T. atroviridae for 5 days. Treating cells with T. harzianum for 15 days afforded maximum synthesis and leaching (8.1 mg/L) of ginsenoside Rh1.