Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle

• Published in: Journal of applied physiology (1985) Vol. 98; no. 5; pp. 1745 - 1752
• Main Authors: Yang, Yifan, Creer, Andrew, Jemiolo, Bozena, Trappe, Scott
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01.05.2005; American Physiological Society
• Subjects: Adult; Biological and medical sciences; Exercise; Exercise - physiology; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation - physiology; Humans; Male; Metabolism; Muscle Proteins - biosynthesis; Muscle Proteins - genetics; Muscle, Skeletal - metabolism; Muscular system; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Time Factors; Transcriptional Activation; Physical exercise; Human; HKII; MyoD; myogenin; Acute; Gene expression; Striated muscle; FAT/CD36; Vertebrata; Mammalia; CPT1; MRF4; Fat; PDK4; Myf5
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.01185.2004

Human Performance Laboratory, Ball State University, Muncie, Indiana Submitted 21 October 2004 ; accepted in final form 17 December 2004 The aim of this study was to examine the time course activation of select myogenic (MRF4, Myf5, MyoD, myogenin) and metabolic (CD36, CPT1, HKII, and PDK4) genes after an acute bout of resistance (RE) or run (Run) exercise. Six RE subjects [25 ± 4 yr (mean ± SD), 74 ± 14 kg, 1.71 ± 0.11 m] and six Run subjects (25 ± 4 yr, 72 ± 5 kg, 1.81 ± 0.07 m, 63 ± 8 ml·kg –1 ·min –1 ) were studied. Eight muscle biopsies were taken from the vastus lateralis (RE) and gastrocnemius (Run) before, immediately after, and 1, 2, 4, 8, 12 and 24 h after exercise. RE increased mRNA of MRF4 (3.7- to 4.5-fold 2–4 h post), MyoD (5.8-fold 8 h post), myogenin (2.6- and 3.5-fold 8–12 h post), HKII (3.6- to 10.5-fold 2–12 h post), and PDK4 (14- to 26-fold 2–8 h post). There were no differences in Myf5, CD36, and CPT1 mRNA levels 0–24 h post-RE. Run increased mRNA of MyoD (5.0- to 8.0-fold), HKII (12- to 16-fold), and PDK4 (32- to 52-fold) at 8–12 h postexercise. There were no differences in MRF4, Myf5, myogenin, CD36 and CPT1 mRNA levels 0–24 h post-Run. These data indicate a myogenic and metabolic gene induction with RE and Run exercise. The timing of the gene induction is variable and generally peaks 4–8 h postexercise with all gene expression not significantly different from the preexercise levels by 24 h postexercise. These data provide basic information for the timing of human muscle biopsy samples for gene-expression studies involving exercise. MRF4; Myf5; MyoD; myogenin; FAT/CD36; CPT1; HKII; PDK4 Address for reprint requests and other correspondence: S. Trappe, Human Performance Laboratory, Ball State Univ., Muncie, IN 47306 (E-mail: strappe{at}bsu.edu )