Occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs

• Published in: Journal of antimicrobial chemotherapy Vol. 60; no. 4; pp. 775 - 781
• Main Authors: Pedersen, Karl, Pedersen, Kristina, Jensen, Helene, Finster, Kai, Jensen, Vibeke F., Heuer, Ole E.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.10.2007; Oxford Publishing Limited (England)
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacteria; Biological and medical sciences; Chemotherapy; companion animals; Dog Diseases - microbiology; Dogs; Drug resistance; Drug Resistance, Bacterial; Drug Utilization - statistics & numerical data; Escherichia coli; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - microbiology; Gram-Positive Bacteria - drug effects; Gram-Positive Bacteria - isolation & purification; Gram-Positive Bacterial Infections - microbiology; Infections; Medical sciences; Microbial Sensitivity Tests; Pasteurella multocida; Pharmacology. Drug treatments; Pseudomonas aeruginosa; pyoderma; Staphylococcus intermedius; Streptococcus canis; companion animals; pyoderma; Staphylococcus intermedius; Fissipedia; Carnivora; Skin disease; Drug susceptibility test; Escherichia coli; Antimicrobial agent; Resistance; Streptococcaceae; Vertebrata; Mammalia; Pyoderma; Animal; Streptococcus canis; Micrococcales; Bacteria; Micrococcaceae; Diagnosis; Veterinary; Dog; Streptococcus G; Enterobacteriaceae
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkm269

Objectives To study the occurrence of antimicrobial resistance among common bacterial pathogens from dogs and relate resistance patterns to data on consumption of antimicrobials. Methods The antimicrobial susceptibility patterns of 201 Staphylococcus intermedius, 37 Streptococcus canis, 39 Pseudomonas aeruginosa, 25 Pasteurella multocida, 29 Proteus spp. and 449 Escherichia coli isolates from clinical submissions from dogs were determined by a broth-dilution method for determination of minimal inhibitory concentration. Data for consumption of antimicrobials were retrieved from VetStat, a national database for reporting antimicrobial prescriptions. Results The majority of the antimicrobials prescribed for dogs were broad-spectrum compounds, and extended-spectrum penicillins, cephalosporins and sulphonamides + trimethoprim together accounted for 81% of the total amount used for companion animals. Resistance to cephalosporins and amoxicillin with clavulanic acid was very low for all bacterial species examined, except for P. aeruginosa, and resistance to sulphonamides and trimethoprim was low for most species. Among the S. intermedius isolates, 60.2% were resistant to penicillin, 30.2% to fusidic acid and 27.9% to macrolides. Among E. coli isolates, the highest level of resistance was recorded for ampicillin, sulphonamides, trimethoprim, tetracyclines and streptomycin. Certain differences in resistance patterns between isolates from different sites or organs were noticed for E. coli, S. intermedius and Proteus isolates. Conclusions This investigation provided data on occurrence of antimicrobial resistance in important pathogenic bacteria from dogs, which may be useful for the small animal practitioner. Resistance was low to the compounds that were most often used, but unfortunately, these compounds were broad-spectrum. Data on resistance and usage may form a background for the establishment of a set of recommendations for prudent use of antimicrobials for companion animals.