Polycyclic aromatic hydrocarbons storage by Fusarium solani in intracellular lipid vesicles

• Published in: Environmental pollution (1987) Vol. 133; no. 2; pp. 283 - 291
• Main Authors: Verdin, Anthony, Lounès-Hadj Sahraoui, Anissa, Newsam, Ray, Robinson, Gary, Durand, Roger
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2005; Elsevier
• Subjects: analysis; Animal, plant and microbial ecology; Applied ecology; Azo Compounds; Azo Compounds - analysis; Benzo[ a]pyrene; benzopyrene; benzopyrenes; bioaccumulation; Biodegradation, Environmental; Biological and medical sciences; Biological Transport; Biological Transport - drug effects; Culture Media; Cytoskeleton; Cytoskeleton - drug effects; drug effects; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; Fusarium; Fusarium - drug effects; Fusarium - metabolism; Fusarium - physiology; Fusarium solani; General aspects; Glucose; Glucose - metabolism; Life Sciences; lipid bodies; Lipid Metabolism; Lipid vesicle; metabolism; PAH storage; pharmacokinetics; physiology; polluted soils; Polycyclic aromatic hydrocarbon; polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - pharmacokinetics; soil fungi; soil pollution; Temperature; uptake mechanisms; PAH storage; Benzo[ a]pyrene; Fusarium solani; Lipid vesicle; Polycyclic aromatic hydrocarbon; Benzopyrene; Hydrocarbon; Lipids; Polycyclic aromatic compound; Fungi; Storage; Benzo[a]pyrene; Fungi Imperfecti; Intracellular; Organic compounds; Thallophyta
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2004.05.040

Accumulation and elimination of polycyclic aromatic hydrocarbons (PAHs) were studied in the fungus Fusarium solani. When the fungus was grown on a synthetic medium containing benzo[ a]pyrene, hyphae of F. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: Sudan III and Rhodamine B. The fluorescence produced by Rhodamine B and PAH benzo[ a]pyrene were at the same locations in the fungal hyphae, indicating that F. solani stored PAH in pre-existing lipid vesicles. A passive temperature-independent process is involved in the benzo[ a]pyrene uptake and storage. Sodium azide, a cytochrome c oxidation inhibitor, and the two cytoskeleton inhibitors colchicine and cytochalasin did not prevent the transport and accumulation of PAH in lipid vesicles of F. solani hyphae. F. solani degraded a large range of PAHs at different rates. PAH intracellular storage in lipid vesicles was not necessarily accompanied by degradation and was common to numerous other fungi. Fungi can store PAHs intracellularly in lipid vesicles independently of their PAH degradation abilities.