Green synthesis of silver/montmorillonite/chitosan bionanocomposites using the UV irradiation method and evaluation of antibacterial activity

• Published in: International journal of nanomedicine Vol. 5; no. default; pp. 875 - 887
• Main Authors: Shameli, Kamyar, Ahmad, Mansor Bin, Yunus, Wan Md Zin Wan, Rustaiyan, Abdolhossein, Ibrahim, Nor Azowa, Zargar, Mohsen, Abdollahi, Yadollah
• Format: Journal Article
• Language: English
• Published: New Zealand Taylor & Francis Ltd 22.10.2010; Dove Press; Dove Medical Press
• Subjects: Anti-Bacterial Agents - administration & dosage; antibacterial activity; Bentonite - chemistry; bionanocomposites; chitosan; Chitosan - chemistry; Disk Diffusion Antimicrobial Tests; Escherichia coli; Escherichia coli - drug effects; Gram-positive bacteria; Green Chemistry Technology - methods; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Methicillin-Resistant Staphylococcus aureus - drug effects; Microscopy; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Models, Molecular; Molecular Structure; montmorillonite; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Nanomedicine; Original Research; Powder Diffraction; Silver; Silver - administration & dosage; Silver - chemistry; silver nanoparticles; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Staphylococcus aureus - drug effects; ultraviolet irradiation; Ultraviolet radiation; Ultraviolet Rays; antibacterial activity; chitosan; montmorillonite; ultraviolet irradiation; bionanocomposites; silver nanoparticles
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S13632

In this study, silver nanoparticles (Ag-NPs) were synthesized using a green physical synthetic route into the lamellar space of montmorillonite (MMT)/chitosan (Cts) utilizing the ultraviolet (UV) irradiation reduction method in the absence of any reducing agent or heat treatment. Cts, MMT, and AgNO(3) were used as the natural polymeric stabilizer, solid support, and silver precursor, respectively. The properties of Ag/MMT/Cts bionanocomposites (BNCs) were studied as the function of UV irradiation times. UV irradiation disintegrated the Ag-NPs into smaller sizes until a relatively stable size and size distribution were achieved. Meanwhile, the crystalline structure and d-spacing of the MMT interlayer, average size and size distribution, surface morphology, elemental signal peaks, functional groups, and surface plasmon resonance of Ag/MMT/Cts BNCs were determined by powder X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray fluorescence, Fourier transform infrared, and UV-visible spectroscopy. The antibacterial activity of Ag-NPs in MMT/Cts was investigated against Gram-positive bacteria, ie, Staphylococcus aureus and methicillin-resistant S. aureus and Gram-negative bacteria (ie, Escherichia coli) by the disk diffusion method on Muller-Hinton Agar at different sizes of Ag-NPs. All of the synthesized Ag/MMT/Cts BNCs were found to have high antibacterial activity. These results show that Ag/MMT/Cts BNCs can be useful in different biologic research and biomedical applications, such as surgical devices and drug delivery vehicles.