HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses

• Published in: Virology (New York, N.Y.) Vol. 322; no. 1; pp. 182 - 189
• Main Authors: Rollman, Erik, Arnheim, Lisen, Collier, Brian, Öberg, Daniel, Hall, Håkan, Klingström, Jonas, Dillner, Joakim, Pastrana, Diana V, Buck, Chris B, Hinkula, Jorma, Wahren, Britta, Schwartz, Stefan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.04.2004
• Subjects: Animals; Antibodies, Viral - blood; Antibodies, Viral - immunology; Basic Medicine; Capsid Proteins; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - immunology; Cells, Cultured; Disease Models, Animal; DNA vaccination; Genes, Regulator; Genes, Viral; HPV-16; Human papillomavirus 16; Imiquimod; Lymphocyte Activation; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Mice; Mice, Inbred C57BL; Microbiology in the medical area; Mikrobiologi inom det medicinska området; Neutralization; Neutralization Tests; Oncogene Proteins, Viral - genetics; Oncogene Proteins, Viral - immunology; Papillomaviridae - genetics; Papillomaviridae - immunology; Papillomavirus Infections - blood; Papillomavirus Infections - immunology; Papillomavirus Infections - prevention & control; Papillomavirus Infections/blood/immunology/prevention & control; Point Mutation; rGM-CSF; Spleen - immunology; T lymphocytes; Tumor immunity; Vaccination; Vaccines, DNA - administration & dosage; T lymphocytes; rGM-CSF; DNA vaccination; Tumor immunity; Imiquimod; HPV-16; Neutralization; Disease Models; Research Support; Genes; Viral; Antibodies; Oncogene Proteins; Regulator; Vaccines; Cells; Inbred C57BL; Viral/genetics/immunology; DNA/administration & dosage; Papillomavirus; Human/genetics/immunology; Viral/blood/immunology; Animal; Cultured; Mice; Non-U.S. Gov't
• ISSN: 0042-6822; 1096-0341; 1096-0341
• DOI: 10.1016/j.virol.2004.02.002

Introduction of point mutations in the 5′ end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57Bl/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.