NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence

The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND‐INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacti...

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Published inPlant direct Vol. 7; no. 9; pp. e529 - n/a
Main Authors Nagahage, Isura Sumeda Priyadarshana, Matsuda, Kohei, Miyashita, Kyoko, Fujiwara, Sumire, Mannapperuma, Chanaka, Yamada, Takuya, Sakamoto, Shingo, Ishikawa, Toshiki, Nagano, Minoru, Ohtani, Misato, Kato, Ko, Uchimiya, Hirofumi, Mitsuda, Nobutaka, Kawai‐Yamada, Maki, Demura, Taku, Yamaguchi, Masatoshi
Format Journal Article
LanguageEnglish
Published England John Wiley & Sons, Inc 01.09.2023
John Wiley and Sons Inc
Wiley
Subjects
Arabidopsis thaliana
Biosynthesis
Chloroplasts
Circadian rhythm
Cloning
Gene expression
leaf senescence
Leaves
Mutants
NAC domain protein
Phenotypes
Proteins
protein–protein interaction
Senescence
Transcription activation
transcription factor
Transcription factors
Xylem
Yeast
Arabidopsis thaliana
protein–protein interaction
transcription factor
NAC domain protein
leaf senescence
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Summary:The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND‐INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two‐hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.
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ISSN:2475-4455
2475-4455
DOI:10.1002/pld3.529