Fortifying angiogenic efficacy of conditioned media using phototoxic‐free blue light for wound healing

We used a blue organic light‐emitting diode (bOLED) to increase the paracrine factors secreted from human adipose‐derived stem cells (hADSCs) for producing conditioned medium (CM). Our results showed that while the bOLED irradiation promotes a mild‐dose reactive oxygen generation that enhances the a...

Full description

Saved in:
Bibliographic Details
Published inBioengineering & translational medicine Vol. 8; no. 3; pp. e10462 - n/a
Main Authors Kim, Sung‐Won, Im, Gwang‐Bum, Kim, Yeong Hwan, Bhang, Suk Ho
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.05.2023
Wiley
Subjects
angiogenesis
Apoptosis
bOLED
conditioned medium
Energy
Gene expression
Growth factors
Heat shock proteins
Hypoxia
Light emitting diodes
Nanomaterials
Nanoparticles
Organic light emitting diodes
phototoxicity
Radiation dosage
ROS
Stains & staining
Stem cells
Toxicity
Wound healing
conditioned medium
phototoxicity
bOLED
angiogenesis
ROS
Online AccessGet full text

Cover

More Information
Summary:We used a blue organic light‐emitting diode (bOLED) to increase the paracrine factors secreted from human adipose‐derived stem cells (hADSCs) for producing conditioned medium (CM). Our results showed that while the bOLED irradiation promotes a mild‐dose reactive oxygen generation that enhances the angiogenic paracrine secretion of hADSCs, it does not induce phototoxicity. The bOLED enhances paracrine factors via a cell‐signaling mechanism involving hypoxia‐inducible factor 1 alpha. This study demonstrated that the CM resulting from bOLED treatment shows improved therapeutic effects on mouse wound‐healing models. This method contributes to overcoming the barriers to stem‐cell therapies, including the toxicity and low yields from other methods such as nanoparticles, synthetic polymers, and even cell‐derived vesicles.
Bibliography:Sung‐Won Kim and Gwang‐Bum Im contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2380-6761
2380-6761
DOI:10.1002/btm2.10462