Crystal structure of barley agmatine coumaroyltransferase, an N‐acyltransferase from the BAHD superfamily

• Published in: Acta crystallographica. Section F, Structural biology communications Vol. 76; no. 12; pp. 590 - 596
• Main Authors: Yamane, Miyo, Takenoya, Mihoko, Yajima, Shunsuke, Sue, Masayuki
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.12.2020; Wiley Subscription Services, Inc
• Subjects: Acyltransferase; Agmatine; agmatine coumaroyltransferase; BAHD superfamily; Barley; Biosynthesis; Crystal structure; Crystals; Enzymes; Hordeum vulgare; Metabolism; Metabolites; N‐acyltransferases; Research Communications; Secondary metabolites; Hordeum vulgare; N-acyltransferases; BAHD superfamily; agmatine coumaroyltransferase
• ISSN: 2053-230X; 2053-230X
• DOI: 10.1107/S2053230X20014880

The enzymes of the BAHD superfamily, a large group of acyl‐CoA‐dependent acyltransferases in plants, are involved in the biosynthesis of diverse secondary metabolites. While the structures of several O‐acyltransferases from the BAHD superfamily, such as hydroxycinnamoyl‐CoA shikimate hydroxycinnamoyl transferase, have been elucidated, no structural information on N‐acyltransferases is available. Hordeum vulgare agmatine coumaroyltransferase (HvACT) is an N‐acyltransferase from the BAHD superfamily and is one of the most important enzymes in the secondary metabolism of barley. Here, an apo‐form structure of HvACT is reported as the first structure of an N‐acyltransferase from the BAHD superfamily. HvACT crystals diffracted to 1.8 Å resolution and belonged to the monoclinic space group P21, with unit‐cell parameters a = 57.6, b = 59.5, c = 73.6 Å, α = 90, β = 91.3 , γ = 90°. Like other known BAHD superfamily structures, HvACT contains two domains that adopt a two‐layer αβ‐sandwich architecture and a solvent‐exposed channel that penetrates the enzyme core. The structure of barley agmatine coumaroyltransferase, a member of the BAHD acyltransferase superfamily, was elucidated. This is the first report of the structure of an N‐acyltransferase from the BAHD superfamily.