DNA microarray technology for simultaneous detection and species identification of seven human herpes viruses

• Published in: Journal of medical virology Vol. 80; no. 6; pp. 1042 - 1050
• Main Authors: Zheng, Zhi-Bei, Wu, Yi-Dong, Yu, Xi-Lin, Shang, Shi-Qiang
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2008; Wiley-Liss
• Subjects: Antibodies, Viral - blood; Biological and medical sciences; Candida albicans; Child; Cryptococcus neoformans; DNA microarrays; DNA-Directed DNA Polymerase - genetics; Epstein-Barr virus; Escherichia coli; Exodeoxyribonucleases - genetics; Fundamental and applied biological sciences. Psychology; Hepatitis B virus; Herpes simplex virus 1; Herpes simplex virus 2; Herpesviridae - classification; Herpesviridae - genetics; Herpesviridae - isolation & purification; Herpesviridae Infections - blood; Herpesviridae Infections - cerebrospinal fluid; Herpesviridae Infections - virology; Herpesvirus; Human cytomegalovirus; human herpes viral infection; Human herpesvirus 6B; Human viral diseases; Humans; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; multiplex PCR; Oligonucleotide Array Sequence Analysis - methods; Reproducibility of Results; Sensitivity and Specificity; Species Specificity; Varicella-zoster virus; Viral diseases; Viral Proteins - genetics; Virology; Infection; Human; multiplex PCR; human herpes viral infection; DNA microarrays; Multiplex polymerase chain reaction; Viral disease; DNA chip; Herpes; Identification; Detection; Species
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.21131

The aim of the study was to develop a multiplex PCR-based DNA microarray technology for simultaneous detection and species identification of seven human herpes viruses, namely herpes simplex virus type 1, type 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpes virus 6 (HHV-6A, HHV-6B), and to apply this technology to accurate diagnosis of herpesvirus-associated diseases. Primers and oligonucleotide probes were designed and synthesized based on the highly conserved regions of the DNA polymerase gene in human herpes viruses. DNA microarrays were made by printing the oligonucleotide probes onto special glass slides. After amplification and labeling with CY5, the PCR products were hybridized with the DNA microarrays and species identified. Sixty-one cerebrospinal fluid (CSF) and 132 blood specimens were analyzed by this technique, and the results were compared with those of TaqMan PCR. Several specimens were sequenced further after cloning. The PCR products of the seven human herpes viruses ranged from 224 to 252 bp, and could be species identified with DNA microarrays. The detection limits were 10¹ copies/μl for each virus. And the test showed no cross-reaction to DNA extracted from S. aureus, E. coli, hepatitis B virus, Cryptococcus neoformans, Candida albicans and human genome. Among 132 blood and 61 CSF specimens, 55 were tested positive for human herpes virus DNA. Compared with the results of TaqMan PCR, the sensitivity and specificity of the DNA microarray technology was 96.2% and 99.3%, respectively. This multiplex PCR-based DNA microarray technology, which is rapid, specific and sensitive, serves as an effective technique for simultaneous detection and species identification of seven human herpes viruses. J. Med. Virol. 80:1042-1050, 2008.