Structure of the (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate reductase from Plasmodium falciparum

• Published in: FEBS letters Vol. 587; no. 24; pp. 3968 - 3972
• Main Authors: Rekittke, Ingo, Olkhova, Elena, Wiesner, Jochen, Demmer, Ulrike, Warkentin, Eberhard, Jomaa, Hassan, Ermler, Ulrich
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 11.12.2013
• Subjects: (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate; (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate reductase; 2-C-methyl-d-erythritol-2,4-cyclo-diphosphate; 2-C-methyl-d-erythritol-4-phosphate; Catalytic Domain; Crystallography, X-Ray; dimethylallyl diphosphate; DMAPP; Drug design; Escherichia coli - enzymology; Escherichia coli - metabolism; Ferredoxins - metabolism; HMBPP; IPP; isopentenyl diphosphate; Isoprenoid biosynthesis; LytB; MEcPP; MEP; Models, Molecular; Molecular Docking Simulation; Organophosphorus Compounds - metabolism; Oxidoreductases - chemistry; Oxidoreductases - metabolism; Plasmodium falciparum; Plasmodium falciparum - enzymology; Plasmodium falciparum - metabolism; Protein Binding; Protein Conformation; Protein Folding; Protozoan Proteins - chemistry; Protozoan Proteins - metabolism; X-ray structure; Plasmodium falciparum; MEcPP; HMBPP; IPP; MEP; DMAPP; Drug design; Isoprenoid biosynthesis; X-ray structure; LytB; 2-C-methyl-d-erythritol-2,4-cyclo-diphosphate; 2-C-methyl-d-erythritol-4-phosphate; isopentenyl diphosphate; (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate; (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate reductase; dimethylallyl diphosphate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2013.10.029

•LytB of the malaria pathogen Plasmodium falciparum was structurally characterized.•The substrate and electron donor binding sites serve as targets for a search for antimalarian drugs.•A LytB-[2Fe–2S] ferredoxin complex was modelled by docking calculations. Terpenoid precursor biosynthesis occurs in human and many pathogenic organisms via the mevalonate and 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways, respectively. We determined the X-ray structure of the Fe/S containing (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate reductase (LytB) of the pathogenic protozoa Plasmodium falciparum which catalyzes the terminal step of the MEP pathway. The cloverleaf fold and the active site of P. falciparum LytB corresponds to those of the Aquifex aeolicus and Escherichia coli enzymes. Its distinct electron donor [2Fe–2S] ferredoxin was modeled to its binding site by docking calculations. The presented structural data provide a platform for a rational search of anti-malarian drugs.