2,3-Butanedione monoxime increases sensitivity to Nikkomycin Z in the budding yeast Saccharomyces cerevisiae

• Published in: World journal of microbiology & biotechnology Vol. 22; no. 3; pp. 255 - 260
• Main Authors: Rivera-Molina, Félix E, González-Crespo, Sahily, Cruz, Yasdet Maldonado-De la, Ortiz-Betancourt, José M, Rodríguez-Medina, José R
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.03.2006; Springer; Springer Nature B.V
• Subjects: BDM; Biological and medical sciences; Biotechnology; Chitin; Fundamental and applied biological sciences. Psychology; Microbiology; myo1; Nikkomycin Z; Proteins; Saccharomyces cerevisiae; Yeast; Yeasts; Fungi; Nikkomycin Z; Sensitivity; Yeast; Ascomycetes; Chitin; BDM; myol; Saccharomyces cerevisiae; Thallophyta; myo1; yeast; chitin
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-005-9028-x

Nikkomycin Z (NZ) is a competitive inhibitor of chitin synthase III in the yeast Saccharomyces cerevisiae. Myosin type II-deficient yeast strains (myo1) display a dramatic reduction in growth when chitin synthase III activity is inhibited by NZ, supporting the contention that actomyosin motility plays an important role in maintaining cell wall integrity. A proposed inhibitor of cortical actin polymerization in vitro, 2,3-butanedione monoxime (BDM), also inhibits growth of wild-type yeast strains at a concentration of 20 mM. In this study, we assayed for potential in vivo interplay between BDM-sensitive cell functions and cell wall chitin synthesis by testing for increased sensitivity to NZ during co-treatment with BDM at sub-inhibitory concentrations. Our results show that BDM can increase the sensitivity of yeast cells to Nikkomycin Z.