Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

• Published in: Analytical and bioanalytical chemistry Vol. 408; no. 26; pp. 7265 - 7269
• Main Authors: Bocková, Markéta, Chadtová Song, Xue, Gedeonová, Erika, Levová, Kateřina, Kalousová, Marta, Zima, Tomáš, Homola, Jiří
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2016; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Antibodies; Biochemistry; Biosensors; Blood; Buffers; Characterization and Evaluation of Materials; Chemical Sensing Systems; Chemistry; Chemistry and Materials Science; Equipment and supplies; Female; Food Science; Gold - chemistry; Humans; Laboratory Medicine; Limit of Detection; Male; Mathematical analysis; Membrane proteins; Metal Nanoparticles - chemistry; Methods; Monitoring/Environmental Analysis; Nanoparticles; Paper in Forefront; Photonics; Physiological aspects; Plasma; Plasmons; Preeclampsia; Pregnancy; Pregnancy-Associated Plasma Protein-A - analysis; Proteins; Referencing; Sensors; Surface Plasmon Resonance - methods; Surface plasmon resonance spectroscopy; Women; United States > US; Czech Republic; Blood sample; Nanoparticles; Surface plasmon resonance; Pregnancy associated plasma protein A2
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-016-9664-z

Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer