Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

• Published in: International journal of nanomedicine Vol. 12; pp. 979 - 987
• Main Authors: Zhu, Xuena, Sarwar, Mehenur, Yue, Qiaoli, Chen, Chunying, Li, Chen-Zhong
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2017; Taylor & Francis Ltd; Dove Medical Press
• Subjects: 8-OHdG; beta-Fructofuranosidase - chemistry; beta-Fructofuranosidase - metabolism; Biological markers; Biomarkers; Biomarkers - analysis; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensor; Biosensors; Blood Glucose Self-Monitoring - instrumentation; Chemical engineering; Chemistry; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - analysis; Deoxyribonucleic acid; Diabetes; DNA; DNA Damage; DNA oxidative damage; Enzymes; Equipment Design; Glucose; Glucose - metabolism; Guanosine - analogs & derivatives; Guanosine - chemistry; Health aspects; Identification and classification; Immunoglobulins; Immunostrip; Innovations; Laboratories; Medical equipment; Monitoring systems; Nanomaterials; Nanoparticles; Nanotechnology; Original Research; Oxidation-Reduction; Oxidative stress; POCTs; Point-of-care; Quantitative analysis; Quantum dots; Smartphone; Smartphones; Sucrose; United States > US; immunostrip; DNA oxidative damage; biosensor; point-of-care; 8-OHdG; POCTs
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S125437

Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL with a dynamic range of 1-200 ng mL . Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.