Effects of the Vitamin D3 on Alleviating the Oxidative Stress Induced by Diquat in Wenchang Chickens

• Published in: Animals (Basel) Vol. 13; no. 4; p. 711
• Main Authors: Nong, Keyi, Liu, Youming, Fang, Xin, Qin, Xinyun, Liu, Zhineng, Zhang, Haiwen
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2023; MDPI
• Subjects: Alanine; Alanine transaminase; Albumins; Analysis; Animals; antioxidant; Antioxidants; Aspartate transaminase; biochemical blood indexed; Body weight loss; broiler; Chickens; Cholesterol; Computer industry; Creatinine; Cytokines; Diamines; Diquat; Enzymatic activity; Enzyme activity; Feed additives; Feeds; Food additives; Inflammation; Interleukin 10; Interleukin 6; intestinal health; Intestine; Jejunum; Kidneys; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Liver; Livestock; Malondialdehyde; Metabolism; Metabolites; Morphology; Muscles; Nutrition research; Oxidation; Oxidative stress; Permeability; Poultry; Poultry feed; Poultry industry; production performance; Slaughter; Triglycerides; Urea; Vitamin D3; Vitamins; Weight loss; Weight reduction; United States; China; Beijing China; Vitamin D3; antioxidant; intestinal health; biochemical blood indexed; production performance; broiler
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani13040711

Vitamin D (VD ) is an indispensable micronutrient in livestock and poultry feed. Its function in antioxidant stress has been reported. We investigate whether the addition of different concentrations of VD to the diet affects the production performance, slaughter performance, meat quality, organ index, and gut injury on the diquat (DQ)-induced model of oxidative stress in Wenchang chickens. Four hundred and eighty one-day-old chickens were randomly divided into six groups: control (basal diet), 4000 VD (basal diet + VD 4000 IU per kg feed intake), 1000 VD+DI (DQ, basal diet + VD 1000 IU per kg feed intake), 2000 VD+DI (DQ, basal diet + VD 2000 IU per kg feed intake), and 4000 VD+DI (DQ, basal diet + VD 4000 IU per kg feed intake). The results showed that the addition of VD to the diet promoted DQ-induced weight loss and reduced ADFI, slaughter rate, splenic index, and pH after 1 h and 24 h in the leg muscles. VD decreased the increase in content of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) among proinflammatory cytokines ( 0.05) and increased the reduction in anti-inflammatory cytokines content of interleukin-10 (IL-10) ( 0.05) induced by DQ. In addition, liver and kidney injury biomarkers and the intestinal permeability index in serum were disordered after treatment with DQ ( 0.05). VD perfected the increase of D-lactic acid (D-LA), diamine oxidase (DAO), total cholesterol (T-CHO), creatinine (CR), blood urea nitrogen (BUN), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) content, aspartate transaminase (AST), alanine transaminase (ALT), and lactate dehydrogenase (LDH) activity ( 0.05); it increased the decrease of albumin (ALB) content ( 0.05). Meanwhile, VD regulated the intestinal morphology and intestinal barrier. Moreover, DQ induced a decrease in total antioxidant capacity and antioxidant enzyme activity in the serum, liver, and jejunum ( 0.05), and an increase in malonaldehyde (MDA) content ( 0.05). However, the addition of different levels of VD could alleviate the above phenomenon of oxidative stress in Wenchang chickens to different degrees. Thus, this research suggested that the addition of VD can relieve the DQ-induced oxidative stress of Wenchang chickens, and the level of VD acquisition is positively correlated with the remission effect.