Efficacy of a Gal-lectin subunit vaccine against experimental Entamoeba histolytica infection and colitis in baboons ( Papio sp.)

• Published in: Vaccine Vol. 30; no. 20; pp. 3068 - 3075
• Main Authors: Abd Alla, Mohamed D, Wolf, Roman, White, Gary L, Kosanke, Stanley D, Cary, David, Verweij, Jaco J, Zhang, Mie-Jie, Ravdin, Jonathan I
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 26.04.2012
• Subjects: Adjuvants, Immunologic - administration & dosage; Allergy and Immunology; Animals; antibodies; Antibodies, Protozoan - blood; Antigens, Protozoan - immunology; Baboon model; cecum; cholera toxin; Cholera Toxin - administration & dosage; colitis; colonoscopy; Disease Models, Animal; Dysentery, Amebic - prevention & control; Entamoeba histolytica; Entamoeba histolytica - immunology; Entamoebiasis - prevention & control; Enzyme-Linked Immunosorbent Assay; feces; Feces - parasitology; histopathology; immunoglobulin A; Lectins - immunology; nose; Papio; parasites; Peptide vaccine; Polymerase Chain Reaction; Primates; Protozoan Vaccines - administration & dosage; Protozoan Vaccines - immunology; synthetic peptides; trophozoites; vaccines; Vaccines, Subunit - administration & dosage; Vaccines, Subunit - immunology; Baboon model; Entamoeba histolytica; Peptide vaccine
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.02.066

Highlights ► Developed a new experimental model of E. histolytica infection in baboons. ► E. histolytica infection and inflammatory colitis was sustained for 90 days. ► A Gal-lectin-based intranasal synthetic peptide vaccine was efficacious. ► Vaccine efficacy was validated by fecal PCR, colonoscopy and pathology. ► Immunity correlated with the ELISA OD value for intestinal anti-lectin IgA.