Sphingomonas sediminicola Is an Endosymbiotic Bacterium Able to Induce the Formation of Root Nodules in Pea ( Pisum sativum L.) and to Enhance Plant Biomass Production

• Published in: Microorganisms (Basel) Vol. 11; no. 1; p. 199
• Main Authors: Mazoyon, Candice, Hirel, Bertrand, Pecourt, Audrey, Catterou, Manuella, Gutierrez, Laurent, Sarazin, Vivien, Dubois, Fréderic, Duclercq, Jérôme
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2023; MDPI
• Subjects: Agricultural practices; Agricultural production; Agriculture; Bacteria; Bacteroids; Biodiversity; Biomass; Cell differentiation; Crop rotation; Experiments; Fertilization; Fertilizers; Genomes; Inoculation; Legumes; Life Sciences; Mineral fertilizers; Nitrogenase; nodulation; Nodules; Peas; PGRP; Phylogeny; Pisum sativum; Plant biomass; Plant growth; Plant resistance; rhizobacteria; Root nodules; Seeds; Soil fertility; Sphingomonas; Sphingomonas sediminicola; Sustainable agriculture; Sustainable development; Sustainable practices; Vegetal Biology; France; United States > US; PGRP; Sphingomonas sediminicola; rhizobacteria; nodulation; Pisum sativum
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11010199

The application of bacterial bio-inputs is a very attractive alternative to the use of mineral fertilisers. In ploughed soils including a crop rotation pea, we observed an enrichment of bacterial communities with ( .) . Inoculation experiments, cytological studies, and sequencing were used to investigate the beneficial role of in pea. is able to colonise pea plants and establish a symbiotic association that promotes plant biomass production. Sequencing of the genome revealed the existence of genes involved in secretion systems, Nod factor synthesis, and nitrogenase activity. Light and electron microscopic observations allowed us to refine the different steps involved in the establishment of the symbiotic association, including the formation of infection threads, the entry of the bacteria into the root cells, and the development of differentiated bacteroids in root nodules. These results, together with phylogenetic analysis, demonstrated that is a non-rhizobia that has the potential to develop a beneficial symbiotic association with a legume. Such a symbiotic association could be a promising alternative for the development of more sustainable agricultural practices, especially under reduced N fertilisation conditions.