tetravalent dengue vaccine containing a mix of domain III-P64k and domain III-capsid proteins induces a protective response in mice

• Published in: Archives of virology Vol. 159; no. 10; pp. 2597 - 2604
• Main Authors: Izquierdo, Alienys, García, Angélica, Lazo, Laura, Gil, Lázaro, Marcos, Ernesto, Alvarez, Mayling, Valdés, Iris, Hermida, Lisset, Guillén, Gerardo, Guzmán, María G
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 01.10.2014; Springer Vienna; Springer Nature B.V
• Subjects: Animals; Antibodies; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Antigens; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Biomedical and Life Sciences; Biomedicine; Capsid Proteins - genetics; Capsid Proteins - immunology; cell-mediated immunity; Cells, Cultured; coat proteins; dengue; Dengue - immunology; Dengue - prevention & control; Dengue - virology; Dengue fever; Dengue Vaccines - immunology; Dengue virus; Dengue Virus - classification; Dengue Virus - immunology; Dengue virus type 2; Disease Models, Animal; Female; Immunization; immunoglobulin G; Immunoglobulin G - immunology; Infections; Infectious Diseases; Interferon-gamma - secretion; Laboratory animals; Medical Microbiology; Mice; Mice, Inbred BALB C; Neisseria meningitidis; Neisseria meningitidis - genetics; Neisseria meningitidis - immunology; neutralizing antibodies; Original Article; Proteins; recombinant fusion proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; secretion; serotypes; Vaccines; Vaccines, Synthetic - immunology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Virology; Viruses; Cuba; Germany; Angola; Neutralize Antibody Titer; Baby Hamster Kidney; Negative Control Group; Dengue Virus; Positive Control Group
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-014-2115-2

Recombinant fusion proteins containing domain III of the dengue virus envelope protein fused to the P64k protein from Neisseria meningitidis and domain III of dengue virus type 2 (D2) fused to the capsid protein of this serotype were immunogenic and conferred protection in mice against lethal challenge, as reported previously. Combining the domain III-P64k recombinant proteins of dengue virus types 1, 3 and 4 (D1, D3, and D4) with the domain III-capsid protein from D2, we obtained a novel tetravalent formulation containing different antigens. Here, the IgG and neutralizing antibody response, the cellular immune response, and the protective capacity against lethal challenge in mice immunized with this tetravalent formulation were evaluated. The neutralizing antibody response obtained against D1, D2 and D3, together with the high levels of IFNγ secretion induced after stimulation with the four dengue serotypes, supports the strategy of using a new tetravalent formulation containing domain III of the envelope protein fused to the capsid protein of each dengue virus serotype.