PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice

Phased small-interfering RNAs (phasiRNAs) are a special class of small RNAs, which are generated in 21- or 24-nt intervals from transcripts of precursor RNAs. Although phasiRNAs have been found in a range of organisms, their biological functions in plants have yet to be uncovered. Here we show that...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 113; no. 52; pp. 15144 - 15149
Main Authors Fan, Yourong, 范优荣, Yang, Jiangyi, 杨江义, Mathioni, Sandra M., Yu, Jinsheng, 於金生, Shen, Jianqiang, 沈建强, Yang, Xuefei, 杨雪菲, Wang, Lei, 王磊, Zhang, Qinghua, 张清华, Cai, Zhaoxia, 蔡朝霞, Xu, Caiguo, 徐才国, Li, Xianghua, 李香花, Xiao, Jinghua, 肖景华, Meyers, Blake C., Zhang, Qifa, 张启发
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 27.12.2016
Subjects
Biological Sciences
Fertility
Male sterility
Plant breeding
Polymorphism
Ribonucleic acid
Rice
RNA
long-noncoding RNA
phasiRNA
photoperiod-sensitive male sterility
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Summary:Phased small-interfering RNAs (phasiRNAs) are a special class of small RNAs, which are generated in 21- or 24-nt intervals from transcripts of precursor RNAs. Although phasiRNAs have been found in a range of organisms, their biological functions in plants have yet to be uncovered. Here we show that phasiRNAs generated by the photopheriod-sensetive genic male sterility 1 (Pms1) locus were associated with photoperiod-sensitive male sterility (PSMS) in rice, a germplasm that started the two-line hybrid rice breeding. The Pms1 locus encodes a long-noncoding RNA PMS1T that was preferentially expressed in young panicles. PMS1T was targeted by miR2118 to produce 21-nt phasiRNAs that preferentially accumulated in the PSMS line under long-day conditions. A single nucleotide polymorphism in PMS1T nearby the miR2118 recognition site was critical for fertility change, likely leading to differential accumulation of the phasiRNAs. This result suggested possible roles of phasiRNAs in reproductive development of rice, demonstrating the potential importance of this RNA class as regulators in biological processes.
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3Present address: School of Agriculture and Food Science, The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A&F University, Linan 311300, China.
Contributed by Qifa Zhang, November 21, 2016 (sent for review October 5, 2016; reviewed by Gynheung An and Yaoguang Liu)
Author contributions: Y.F., J. Yang, and Qifa Zhang designed research; Y.F., J. Yang, S.M.M., J. Yu, J.S., X.Y., L.W., Qinghua Zhang, and Z.C. performed research; C.X., X.L., J.X., and B.C.M. contributed new reagents/analytic tools; Y.F., J. Yang, and Qifa Zhang analyzed data; and Y.F., J. Yang, and Qifa Zhang wrote the paper.
Reviewers: G.A., Kyung Hee University; and Y.L., South China Agricultural University.
1Present address: College of Life Science and Technology, Guangxi University, Nanning 530004, China.
2Present address: Donald Danforth Plant Science Center, St. Louis, MO 63132.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1619159114