Bioactivity Screening and Chemical Characterization of Biocompound from Endophytic Neofusicoccum parvum and Buergenerula spartinae Isolated from Mangrove Ecosystem

• Published in: Microorganisms (Basel) Vol. 11; no. 6; p. 1599
• Main Authors: Cadamuro, Rafael Dorighello, Bastos, Isabela Maria Agustini da Silveira, de Freitas, Ana Claudia Oliveira, Rosa, Marilene da Silva, Costa, Geovanna de Oliveira, da Silva, Izabella Thaís, Robl, Diogo, Stoco, Patricia Hermes, Sandjo, Louis Pergaud, Treichel, Helen, Steindel, Mário, Fongaro, Gislaine
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.06.2023; MDPI
• Subjects: antibacterial properties; Assaying; Avicennia schaueriana; Bacteria; Bacterial infections; Biocompatibility; Biological activity; Biomolecules; bioprospection; Buergenerula spartinae; Chromatography; Contamination; Cytotoxicity; Dichloromethane; E coli; Endophytes; endophytic fungi; Environmental conditions; Escherichia coli; Fermentation; Fungi; Fusaproliferin; Gas flow; Gram-positive bacteria; Ions; Laguncularia racemosa; Leishmania; Liquid chromatography; Listeria monocytogenes; mangrove; mangrove ecosystems; Mass spectrometry; Metabolites; methanol; methylene chloride; microbial control; Microorganisms; Neofusicoccum parvum; Parasitic diseases; Pathogens; Phenylalanine; pollution; Public health; Scientific imaging; Solid state fermentation; Toxicity; toxicity testing; Tropical diseases; Trypanosoma; Ultraviolet radiation; Yeast; yeasts; Brazil; St Louis Missouri; United States > US; Latin America; microbial control; bioprospection; mangrove; endophytic fungi
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11061599

The discovery of biomolecules has been the subject of extensive research for several years due to their potential to combat harmful pathogens that can lead to environmental contamination and infections in both humans and animals. This study aimed to identify the chemical profile of endophytic fungi, namely Neofusicoccum parvum and Buergenerula spartinae, which were isolated from Avecinnia schaueriana and Laguncularia racemosa. We identified several HPLC-MS compounds, including Ethylidene-3,39-biplumbagin, Pestauvicolactone A, Phenylalanine, 2-Isopropylmalic acid, Fusaproliferin, Sespendole, Ansellone, Calanone derivative, Terpestacin, and others. Solid-state fermentation was conducted for 14–21 days, and methanol and dichloromethane extraction were performed to obtain a crude extract. The results of our cytotoxicity assay revealed a CC50 value > 500 μg/mL, while the virucide, Trypanosoma, leishmania, and yeast assay demonstrated no inhibition. Nevertheless, the bacteriostatic assay showed a 98% reduction in Listeria monocytogenes and Escherichia coli. Our findings suggest that these endophytic fungi species with distinct chemical profiles represent a promising niche for further exploring new biomolecules.