The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide su...
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| Published in | International journal of nanomedicine Vol. 11; pp. 6267 - 6281 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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New Zealand
Dove Medical Press Limited
01.01.2016
Taylor & Francis Ltd Dove Medical Press |
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| Abstract | Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe
O
) and studied their effect on proliferation and neuronal differentiation.
We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR.
Cell proliferation was not affected by PLL-coated γ-Fe
O
but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe
O
. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles.
Our results show that cells labeled with PLL-coated γ-Fe
O
are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. |
|---|---|
| AbstractList | Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation. Materials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Results: Cell proliferation was not affected by PLL-coated γ-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Conclusion: Our results show that cells labeled with PLL-coated γ-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe O ) and studied their effect on proliferation and neuronal differentiation. We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Cell proliferation was not affected by PLL-coated γ-Fe O but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe O . Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Our results show that cells labeled with PLL-coated γ-Fe O are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. Klára Jiráková,1 Monika Šeneklová,1,2 Daniel Jirák,3,4 Karolína Turnovcová,1 Magda Vosmanská,5 Michal Babič,6 Daniel Horák,6 Pavel Veverka,7 Pavla Jendelová1,2 1Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 2Department of Neuroscience, Second Faculty of Medicine, Charles University, 3MR-Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine, 4Department of Biophysics, Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University, 5Department of Analytical Chemistry, University of Chemistry and Technology, 6Department of Polymer Particles, Institute of Macromolecular Chemistry, 7Department of Magnetics and Superconductors, Institute of Physics, ASCR, Prague, Czech Republic Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation. Materials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Results: Cell proliferation was not affected by PLL-coated γ-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Conclusion: Our results show that cells labeled with PLL-coated γ-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. Keywords: neural precursors, magnetic resonance imaging, cell differentiation, superparamagnetic iron oxide nanoparticles, ferrites Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation.INTRODUCTIONMagnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation.We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR.MATERIALS AND METHODSWe investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR.Cell proliferation was not affected by PLL-coated γ-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles.RESULTSCell proliferation was not affected by PLL-coated γ-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles.Our results show that cells labeled with PLL-coated γ-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.CONCLUSIONOur results show that cells labeled with PLL-coated γ-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders. |
| Audience | Academic |
| Author | Jirak, Daniel Turnovcova, Karolina Vosmanska, Magda Seneklova, Monika Jiráková, Klára Jendelova, Pavla Babic, Michal Horak, Daniel Veverka, Pavel |
| AuthorAffiliation | 7 Department of Magnetics and Superconductors, Institute of Physics, ASCR, Prague, Czech Republic 1 Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic 2 Department of Neuroscience, Second Faculty of Medicine, Charles University 4 Department of Biophysics, Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University 6 Department of Polymer Particles, Institute of Macromolecular Chemistry 3 MR-Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine 5 Department of Analytical Chemistry, University of Chemistry and Technology |
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| Keywords | cell differentiation magnetic resonance imaging neural precursors ferrites superparamagnetic iron oxide nanoparticles |
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| Snippet | Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors... Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural... Klára Jiráková,1 Monika Šeneklová,1,2 Daniel Jirák,3,4 Karolína Turnovcová,1 Magda Vosmanská,5 Michal Babič,6 Daniel Horák,6 Pavel Veverka,7 Pavla Jendelová1,2... |
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| SubjectTerms | Biophysics Brain-derived neurotrophic factor Cell Differentiation Cell growth Cell Proliferation Cells, Cultured Cobalt Contrast Media - chemistry Dopamine Experiments Female Ferric oxide ferrites Fetus - cytology Fibroblasts - cytology Flow Cytometry Growth factors Humans Immunoenzyme Techniques Induced Pluripotent Stem Cells - cytology Iron compounds Laboratories Lung - cytology Lysine Lysine - chemistry magnetic resonance imaging Magnetic Resonance Imaging - methods Magnetite Nanoparticles - chemistry Medical research Medicine Microscopy, Electron, Transmission Nanoparticles Nervous system neural precursors Neurons Neurons - cytology Neurosciences Original Research Real-Time Polymerase Chain Reaction Silicon dioxide Stem cells superparamagnetic iron oxide nanoparticles Transplants & implants |
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| Title | The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors |
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