The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

• Published in: International journal of nanomedicine Vol. 11; pp. 6267 - 6281
• Main Authors: Jiráková, Klára, Seneklova, Monika, Jirak, Daniel, Turnovcova, Karolina, Vosmanska, Magda, Babic, Michal, Horak, Daniel, Veverka, Pavel, Jendelova, Pavla
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2016; Taylor & Francis Ltd; Dove Medical Press
• Subjects: Biophysics; Brain-derived neurotrophic factor; Cell Differentiation; Cell growth; Cell Proliferation; Cells, Cultured; Cobalt; Contrast Media - chemistry; Dopamine; Experiments; Female; Ferric oxide; ferrites; Fetus - cytology; Fibroblasts - cytology; Flow Cytometry; Growth factors; Humans; Immunoenzyme Techniques; Induced Pluripotent Stem Cells - cytology; Iron compounds; Laboratories; Lung - cytology; Lysine; Lysine - chemistry; magnetic resonance imaging; Magnetic Resonance Imaging - methods; Magnetite Nanoparticles - chemistry; Medical research; Medicine; Microscopy, Electron, Transmission; Nanoparticles; Nervous system; neural precursors; Neurons; Neurons - cytology; Neurosciences; Original Research; Real-Time Polymerase Chain Reaction; Silicon dioxide; Stem cells; superparamagnetic iron oxide nanoparticles; Transplants & implants; United States > US; Czech Republic; cell differentiation; magnetic resonance imaging; neural precursors; ferrites; superparamagnetic iron oxide nanoparticles
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S116171

Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe O ) and studied their effect on proliferation and neuronal differentiation. We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. Cell proliferation was not affected by PLL-coated γ-Fe O but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe O . Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. Our results show that cells labeled with PLL-coated γ-Fe O are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.