Oleocanthal Ameliorates Amyloid-β Oligomers Toxicity on Astrocytes and Neuronal Cells: In-vitro Studies

• Published in: Neuroscience Vol. 352; pp. 204 - 215
• Main Authors: Batarseh, Yazan S, Mohamed, Loqman A, Al Rihani, Sweilem B, Mousa, Youssef M, Siddique, Abu Bakar, El Sayed, Khalid A, Kaddoumi, Amal
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 03.06.2017
• Subjects: Aldehydes - pharmacology; Amyloid beta-Peptides - toxicity; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; amyloid-β; Anti-Inflammatory Agents - pharmacology; astrocytes; Astrocytes - drug effects; Cell Line, Tumor; Disks Large Homolog 4 Protein - metabolism; Dose-Response Relationship, Drug; Glutamate Plasma Membrane Transport Proteins - metabolism; Humans; Interleukin-6 - metabolism; Nerve Tissue Proteins - metabolism; neuroinflammation; Neurology; neurons; Neurons - drug effects; oleocanthal; Phenols - pharmacology; Synaptosomal-Associated Protein 25 - metabolism; Time Factors; Transfection; APP; BBB; DMSO; Extra-virgin olive oil; Radioimmunoprecipitation assay; IDE; Blood brain barrier; Insulin-degrading enzyme; RIPA; Aβ oligomers; Interleukin-6; Aβ; BSA; soluble APPβ; Apolipoprotein E; Phosphate buffer saline; ApoE; Amyloid-β; ABCA1; Glucose transporter; PBS; Bovine serum albumin; GLUT1; AD; GLT1; Alzheimer’s disease; Astrocytes; Neurons; Astrocytes conditioned media; Glial fibrillary acidic protein; Aβ monomer; Soluble APPα; Neuroinflammation; ACM; Oleocanthal; Amyloid precursor protein; IL-6; GFAP; sAPPα; EVOO; ATP-binding cassette transporter-A1; Glutamate transporter; Dimethyl sulfoxide; sAPPβ; Aβm; Aβo; neurons; oleocanthal; neuroinflammation; amyloid-β; astrocytes
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2017.03.059

Highlights • Oleocanthal rectified Aβo-induced pathological changes in astrocytes and neurons in-vitro. • Oleocanthal reduced astrocytes activation and inflammation associated with Aβ oligomers exposure. • The neuroprotective effect of oleocanthal could be direct and not mediated by astrocytes.