Synthetic nanoparticle vaccines produced by layer-by-layer assembly of artificial biofilms induce potent protective T-cell and antibody responses in vivo

• Published in: Vaccine Vol. 29; no. 3; pp. 558 - 569
• Main Authors: Powell, Thomas J, Palath, Naveen, DeRome, Mary E, Tang, Jie, Jacobs, Andrea, Boyd, James G
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 10.01.2011; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Animals; Antibodies, Bacterial - blood; Antigen presentation; Applied microbiology; Biofilms; Biological and medical sciences; Calcium Carbonate - administration & dosage; Calcium Carbonate - metabolism; CD8-Positive T-Lymphocytes - immunology; Cross-Priming; Cytotoxicity, Immunologic; Dendritic Cells - immunology; Design; Female; Fundamental and applied biological sciences. Psychology; Immune system; Immunization; Interferon-gamma - secretion; Interleukin-4 - secretion; Listeria monocytogenes; Mice; Mice, Inbred C57BL; Microbiology; Nanoparticle; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Ovalbumin - administration & dosage; Ovalbumin - immunology; Ovalbumin - metabolism; Peptides; Phagocytosis; T cell receptors; T-Lymphocytes - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); poly- l-lysine; Antigen presentation; PLL conjugated to fluorescein isothiocynate; 20 mM Tris base, pH 7.6, 140 mM NaCl, 0.1% Tween 20; layer-by-layer; PGA–TR; Nanoparticle; bone marrow dendritic cells; PGA conjugated to Texas red; PGA; Vaccines; DP; PLL; PLL–FITC; TBS/T; BMDC; LbL; ovalbumin; poly- l-glutamic acid; designed peptide; OVA; Antibody; Biofilm; Cellular immunity; Vaccine
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2010.10.001

Abstract Nanoparticle vaccines induce potent immune responses in the absence of conventional adjuvant due to the recognition by immune cells of the particle structures, which mimic natural pathogens such as viruses and bacteria. Nanoparticle vaccines were fabricated by constructing artificial biofilms using layer-by-layer (LbL) deposition of oppositely charged polypeptides and target designed peptides on CaCO3 cores. LbL nanoparticles were efficiently internalized by dendritic cells in vitro by a mechanism that was at least partially phagocytic, and induced DC maturation without triggering secretion of inflammatory cytokines. LbL nanoparticle delivery of designed peptides to DC resulted in potent cross-presentation to CD8+ T-cells and more efficient presentation to CD4+ T-cells compared to presentation of soluble peptide. A single immunization of mice with LbL nanoparticles containing designed peptide induced vigorous T-cell responses characterized by a balanced effector (IFNγ) and Th2 (IL-4) ELISPOT profile and in vivo CTL activity. Mice immunized with LbL nanoparticles bearing ovalbumin-derived designed peptides were protected from challenge with Listeria monocytogenes ectopically expressing ovalbumin, confirming the relevance of the CTL/effector T-cell responses. LbL nanoparticles also elicited antibody responses to the target epitope but not to the matrix components of the nanoparticle, avoiding the vector or carrier affect that hampers utility of other vaccine platforms. The potency and efficacy of LbL nanoparticles administered in aqueous suspension without adjuvant or other formulation additive, and the absence of immune responses to the matrix components, suggest that this strategy may be useful in producing novel vaccines against multiple diseases.