Variation in the tonoplast cadmium transporter heavy metal ATPase 3 (HMA3) homolog gene in Aegilops tauschii

• Published in: PloS one Vol. 18; no. 3; p. e0279707
• Main Authors: Li, Shengke, Li, Xiao, Li, Shijie, Liu, Yu'e, Zang, Tianqing, Hao, Ming, Zhang, Lianquan, Huang, Lin, Jiang, Bo, Yuan, Zhongwei, Chen, Xuejiao, Chen, Xue, Liu, Dengcai, Ning, Shunzong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03.03.2023; Public Library of Science (PLoS)
• Subjects: Adenosine Triphosphatases; Aegilops; Aegilops tauschii; Amino acids; Analysis; Biology and Life Sciences; Bread; Cadmium; Cadmium content; Engineering and Technology; Food safety; Genetic aspects; Genetic diversity; Genetic resources; Genomes; Germplasm; Goat grass; Grain; Haplotypes; Heavy metals; Medicine and Health Sciences; Membrane Transport Proteins; Metals, Heavy; Nucleotides; Phylogenetics; Polymorphism; Polypeptides; Properties; Research and Analysis Methods; Rice; Shoots; Single-nucleotide polymorphism; Transmembrane domains; Triticum; Wheat; China; Canada; Azerbaijan; Turkey; Quebec Canada
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0279707

The functionality of HMA3 is a key determinant controlling Cd accumulation in the shoots and grains of plants. Wild relatives of modern crop plants can serve as sources of valuable genetic variation for various traits. Here, resequencing of HMA3 homoeologous genes from Aegilops tauschii (the donor of the wheat D genome) was carried out to identify natural variation at both the nucleotide and polypeptide levels. HMA3 homoeologs are highly conserved, and 10 haplotypes were revealed based on 19 single nucleotide polymorphisms (eight induced single amino acid residue substitutions, including 2 altered amino acids in transmembrane domains) in 80 widely distributed Ae. tauschii accessions. The results provide genetic resources for low/no Cd concentration wheat improvement.