Nucleotide sequence and promoter analysis of SPO13, a meiosis-specific gene of Saccharomyces cerevisiae

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 87; no. 23; pp. 9406 - 9410
• Main Authors: Buckingham, L.E. (University of Chicago, Chicago, IL), Wang, H.T, Elder, R.T, McCarroll, R.M, Slater, M.R, Esposito, R.E
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.12.1990; National Acad Sciences
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Amino Acid Sequence; Amino acids; Base Sequence; BETA GALACTOSIDASA; BETA GALACTOSIDASE; beta-Galactosidase - genetics; beta-Galactosidase - metabolism; Biological and medical sciences; Cloning, Molecular; CRECIMIENTO; CROISSANCE; DNA, Fungal - genetics; ESPORULACION; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; Genes; Genes, Fungal; Genes. Genome; Genetic mutation; GENETICA; GENETIQUE; LEVADURA; LEVURE; Meiosis; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; NUCLEOTIDE; NUCLEOTIDOS; Open reading frames; Plasmids; Point mutation; Promoter Regions, Genetic; RNA; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - physiology; Sequence Homology, Nucleic Acid; Spores, Fungal - physiology; SPORULATION; Yeasts; Fungi; Yeast; Gene; Nucleotide sequence; Transcription; Transcription promoter; Sporulation; Cell cycle; Ascomycetes; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.87.23.9406

The SPO13 gene, required for meiosis I segregation in Saccharomyces cerevisiae, produces two developmentally regulated transcripts (1.0 and 1.4 kilobases) that differ in length at their 5' ends. The shorter transcript is sufficient to complement the spo13-1 mutation and contains a major open reading frame encoding a highly basic protein of 33.4 kilodaltons. A fragment upstream (-170 to -8) of the open reading frame confers meiosis-specific transcription on a spo13-HIS3 fusion. Deletions at the 5' end of spo13-lacZ fusions define a region between -140 and -80 that is essential for meiosis-specific expression. This region acts in an orientation-independent manner and is responsive to the MAT-RME regulatory cascade. It contains a 10-base-pair sequence, TAGCCGCCGA, found in a number of meiosis-specific genes, that appears to be required for SPO13 expression. This sequence is identical to URS1, a ubiquitous mitotic repressor element.