Humanized Stem Cell Models of Pediatric Medulloblastoma Reveal an Oct4/mTOR Axis that Promotes Malignancy

• Published in: Cell stem cell Vol. 25; no. 6; pp. 855 - 870.e11
• Main Authors: Čančer, Matko, Hutter, Sonja, Holmberg, Karl O., Rosén, Gabriela, Sundström, Anders, Tailor, Jignesh, Bergström, Tobias, Garancher, Alexandra, Essand, Magnus, Wechsler-Reya, Robert J., Falk, Anna, Weishaupt, Holger, Swartling, Fredrik J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.12.2019; Cell Press
• Subjects: Animals; Cell Line; Cell Proliferation - genetics; Cell Proliferation - physiology; Cell Survival - genetics; Cell Survival - physiology; Female; Fluorescent Antibody Technique; Gene Expression Regulation, Neoplastic - genetics; Gene Expression Regulation, Neoplastic - physiology; Immunohistochemistry; Medicin och hälsovetenskap; medulloblastoma; Medulloblastoma - genetics; Medulloblastoma - metabolism; Medulloblastoma - pathology; metastasis; Mice; mTOR; MYCN; neuroepithelial stem cells; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; POU5F1; reprogramming; Signal Transduction - genetics; Signal Transduction - physiology; Stem Cells - metabolism; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism; reprogramming; MYCN; medulloblastoma; metastasis; neuroepithelial stem cells; mTOR; POU5F1
• ISSN: 1934-5909; 1875-9777; 1875-9777
• DOI: 10.1016/j.stem.2019.10.005

Medulloblastoma (MB), the most frequent malignant childhood brain tumor, can arise from cellular malfunctions during hindbrain development. Here we generate humanized models for Sonic Hedgehog (SHH)-subgroup MB via MYCN overexpression in primary human hindbrain-derived neuroepithelial stem (hbNES) cells or iPSC-derived NES cells, which display a range of aggressive phenotypes upon xenografting. iPSC-derived NES tumors develop quickly with leptomeningeal dissemination, whereas hbNES-derived cells exhibit delayed tumor formation with less dissemination. Methylation and expression profiling show that tumors from both origins recapitulate hallmarks of infant SHH MB and reveal that mTOR activation, as a result of increased Oct4, promotes aggressiveness of human SHH tumors. Targeting mTOR decreases cell viability and prolongs survival, showing the utility of these varied models for dissecting mechanisms mediating tumor aggression and demonstrating the value of humanized models for a better understanding of pediatric cancers. [Display omitted] •Human iPSC-derived or primary neuroepithelial stem cells can be transformed by MYCN•MYCN drives infant SHH medulloblastoma with clinically relevant features•Epigenetically regulated Oct4 promotes mTOR hyperactivation in infant SHH tumors•mTOR inhibition efficiently targets metastatic SHH medulloblastoma models and PDXs Čančer et al. report multiple models of infant Shh medulloblastoma; human iPSC-derived models display significant malignancy, whereas primary hindbrain neuroepithelial stem cell-derived models are less aggressive. These models were used to identify a role for Oct4 in promoting mTOR activation in metastatic tumors, which were sensitive to mTOR inhibition.