Increased expression of eotaxin-3 distinguishes between eosinophilic esophagitis and gastroesophageal reflux disease

• Published in: Human pathology Vol. 38; no. 12; pp. 1744 - 1753
• Main Authors: Bhattacharya, Baishali, MD, MPH, Carlsten, James, MD, Sabo, Edmond, MD, Kethu, Sripathi, MD, Meitner, Patricia, PhD, Tavares, Rosemarie, BS, Jakate, Shriram, MD, FRCPath, Mangray, Shamlal, MD, Aswad, Bassam, MD, Resnick, Murray B, MD, PhD
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2007; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Biological and medical sciences; Biomarkers - analysis; Bone marrow; Chemokine CCL26; Chemokine receptor 3 (CCR-3); Chemokines; Chemokines, CC - biosynthesis; Child; Child, Preschool; Diagnosis, Differential; Eosinophilia - diagnosis; Eosinophilia - metabolism; Eosinophilic esophagitis; Eosinophils; Eotaxin; Eotaxin-3; Eotaxins; Esophagitis - diagnosis; Esophagitis - metabolism; Esophagus; Female; Gastroenterology. Liver. Pancreas. Abdomen; Gastroesophageal reflux; Gastroesophageal Reflux - diagnosis; Gastroesophageal Reflux - metabolism; Gene Expression; Humans; Infant; Inflammatory bowel disease; Interleukin-5 (IL-5); Interleukin-5 - biosynthesis; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Middle Aged; Nutrition research; Other diseases. Semiology; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Receptors, CCR3 - biosynthesis; Recruitment; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Gastroesophageal reflux; Eotaxin-3; Eosinophilic esophagitis; Chemokine receptor 3 (CCR-3); Eotaxins; Interleukin-5 (IL-5); Eotaxin; Eosinophils; Cytokine; Esophageal disease; Granulocyte; Esophagitis; Chemokine receptor; CCR3 chemokine receptor; Eosinophil; Chemokine; Anatomic pathology; Interleukin 5; Digestive diseases
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2007.05.008

Summary Differentiating eosinophilic esophagitis from gastroesophageal reflux disease is important given their pathogenetic differences and responses to therapy. Eotaxins are a family of chemokines important for activation and recruitment of eosinophils mediated by their receptor, chemokine receptor-3 (CCR-3). Interleukin 5 (IL-5) is a key cytokine involved in many steps of eosinophil production and recruitment. The aim of this study was to compare the messenger RNA expression of the eotaxins, CCR-3, and IL-5 between well-characterized groups of patients with eosinophilic esophagitis, patients with gastroesophageal reflux disease, and healthy individuals. This was a retrospective study using esophageal biopsies from 33 patients with eosinophilic esophagitis, 20 patients with gastroesophageal reflux disease, and 17 healthy controls. Parameters studied included demographic features, presenting symptoms, endoscopic findings, histopathologic features, and messenger RNA levels of eotaxins 1, 2, and 3, CCR-3, and IL-5 by quantitative real-time polymerase chain reaction using formalin-fixed, paraffin-embedded tissue. Patients with eosinophilic esophagitis were predominantly males (M/F = 3:1), with a mean age of 15.9 years and a mean eosinophil count of 55 per ×400 high-power field. Patients with gastroesophageal reflux disease had a mean age of 31.5 years and a mean eosinophil count of 5.8 per high-power field. Total intraepithelial eosinophil and lymphocyte counts, the presence of superficial eosinophil clusters, microabscesses, and basal cell hyperplasia were all significantly associated with eosinophilic esophagitis as opposed to gastroesophageal reflux disease ( P < .0001). The mean expression levels of eotaxin-3 were markedly elevated in patients with eosinophilic esophagitis as compared with the gastroesophageal reflux disease and healthy control groups (731 ± 276, 31 ± 12, and 1.5 ± 0.4 pg/ng β -actin, respectively; P < .001). Mean expression levels of eotaxins 1 and 2, IL-5, and CCR-3 were also significantly increased in the patients with eosinophilic esophagitis, albeit at lower levels than eotaxin-3. In conclusion, our results highlight the important contribution of eotaxin-3 in the pathogenesis of eosinophilic esophagitis. Determination of eotaxin-3 levels by real-time polymerase chain reaction on paraffinized, formalin-fixed tissue may be a useful test in the differentiation of eosinophilic esophagitis from gastroesophageal reflux disease.