Optimal activation of Fc-mediated effector functions by influenza virus hemagglutinin antibodies requires two points of contact
Influenza virus strain-specific monoclonal antibodies (mAbs) provide protection independent of Fc gamma receptor (FcγR) engagement. In contrast, optimal in vivo protection achieved by broadly reactive mAbs requires Fc–FcγR engagement. Most strain-specific mAbs target the head domain of the viral hem...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 113; no. 40; pp. E5944 - E5951 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
04.10.2016
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Series | PNAS Plus |
Subjects | |
Online Access | Get full text |
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Summary: | Influenza virus strain-specific monoclonal antibodies (mAbs) provide protection independent of Fc gamma receptor (FcγR) engagement. In contrast, optimal in vivo protection achieved by broadly reactive mAbs requires Fc–FcγR engagement. Most strain-specific mAbs target the head domain of the viral hemagglutinin (HA), whereas broadly reactive mAbs typically recognize epitopes within the HA stalk. This observation has led to questions regarding the mechanism regulating the activation of Fc-dependent effector functions by broadly reactive antibodies. To dissect the molecular mechanism responsible for this dichotomy, we inserted the FLAG epitope into discrete locations on HAs. By characterizing the interactions of several FLAG-tagged HAs with a FLAG-specific antibody, we show that in addition to Fc–FcγR engagement mediated by the FLAG-specific antibody, a second intermolecular bridge between the receptor-binding region of the HA and sialic acid on effector cells is required for optimal activation. Inhibition of this second molecular bridge, through the use of an F(ab′)₂ or the mutation of the sialic acid-binding site, renders the Fc–FcγR interaction unable to optimally activate effector cells. Our findings indicate that broadly reactive mAbs require two molecular contacts to possibly stabilize the immunologic synapse and potently induce antibody-dependent cell-mediated antiviral responses: (i) the interaction between the Fc of a mAb bound to HA with the FcγR of the effector cell and (ii) the interaction between the HA and its sialic acid receptor on the effector cell. This concept might be broadly applicable for protective antibody responses to viral pathogens that have suitable receptors on effector cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: P.E.L., W.H., P.P., and G.S.T. designed research; P.E.L., W.H., C.E.M., M.J.B., and G.S.T. performed research; P.E.L., W.H., C.E.M., M.J.B., M.S.M., F.K., P.P., and G.S.T. analyzed data; and P.E.L., W.H., C.E.M., P.P., and G.S.T. wrote the paper. Contributed by Peter Palese, August 15, 2016 (sent for review July 17, 2016; reviewed by Wendy Barclay, Maryna C. Eichelberger, and Anice C. Lowen) Reviewers: W.B., Imperial College; M.C.E., Center for Biologics Evaluation and Research, Food and Drug Administration; and A.C.L., Emory University School of Medicine. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1613225113 |