Correlation analysis of hepatic steatosis and hepatitis B virus: a cross-sectional study

• Published in: Virology journal Vol. 21; no. 1; p. 22
• Main Authors: Yi, Sitong, Ren, Guanghui, Zhu, Ying, Cong, Qingwei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.01.2024; BioMed Central; BMC
• Subjects: Biotechnology; Care and treatment; CHB; Comparative analysis; Correlation analysis; Cross-Sectional Studies; Deoxyribonucleic acid; Diabetes; Diagnosis; DNA; DNA, Viral; Family medical history; Fatty liver; Genetic aspects; Hepatic steatosis; Hepatitis B; Hepatitis B e antigen; Hepatitis B e Antigens; Hepatitis B surface antigen; Hepatitis B Surface Antigens; Hepatitis B virus; Hepatitis B virus - genetics; Hepatitis B, Chronic; Humans; Infections; Liver cancer; Liver cirrhosis; Liver diseases; MAFLD; Metabolism; Patients; pgRNA; Regression analysis; Serology; Statistical analysis; Steatosis; Virology; China; MAFLD; Hepatic steatosis; Hepatitis B virus; pgRNA; CHB
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-023-02277-8

The co-occurrence of chronic hepatitis B (CHB) and metabolic dysfunction-associated fatty liver disease (MAFLD) has drawn considerable attention due to its impact on disease outcomes. This study aimed to investigate the association between hepatic steatosis and hepatitis B virus (HBV) and analyzed the influence of hepatic steatosis on hepatitis B virology in patients with CHB. In this cross-sectional study, 272 patients infected with HBV who were treatment-naïve or had ceased antiviral treatment for > 6 months were categorized into the CHB group (n = 128) and CHB + MAFLD group (n = 144). Furthermore, based on whether HBV DNA was higher than 2000 IU/mL, patients were categorized into the high-level HBV DNA group (n = 129) and the low-level HBV DNA group (n = 143). The impact of hepatic steatosis on hepatitis B virology was analyzed within the CHB cohort. Multivariate logistic regression analysis was employed to identify independent factors influencing pre-genomic RNA (pgRNA) levels below the lower limit of detection (LLD) in patients with CHB. Among the 272 patients, compared with CHB group, HBV DNA levels (4.11 vs. 3.62 log IU/mL, P = 0.045), hepatitis B surface antigen (HBsAg) levels (3.52 vs. 3.20 log IU/mL, P = 0.008) and the hepatitis B e antigen (HBeAg) positive rate (33.6% vs. 22.2%, P = 0.036) were significantly decreased in the CHB + MAFLD group; In 143 low-level HBV DNA patients, the CHB + MAFLD group exhibited decreased levels of pgRNA and HBsAg compared to the CHB group. However, in 129 high-level HBV DNA patients, a more significant decrease was observed in pgRNA (3.85 vs 3.35 log copies/mL, P = 0.044) and HBsAg (3.85 vs 3.59 log IU/mL, P = 0.033); Spearman correlation analysis revealed a negative correlation between hepatic steatosis and pgRNA (r =  - 0.529, P < 0.001), HBV DNA (r =  - 0.456, P < 0.001), HBsAg (r =  - 0.465, P < 0.001) and HBeAg (r =  - 0.339, P < 0.001) levels; Multivariate logistic regression analysis identified HBV DNA (odds ratio [OR] = 0.283, P < 0.001), HBsAg (OR = 0.300, P < 0.001), and controlled attenuation parameter (CAP) values (OR = 1.013, P = 0.038) as independent factors influencing pgRNA levels below the LLD in patients with CHB. This study establishes a negative correlation between hepatic steatosis and hepatitis B virology, demonstrating decreased HBV expression in patients with CHB + MAFLD.