Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains

• Published in: BioMed research international Vol. 2021; pp. 6662027 - 11
• Main Authors: Soloveva, Irina V., Ilyicheva, Tatyana N., Marchenko, Vasiliy Yu, Pyankov, Oleg V., Tochilina, Anna G., Belova, Irina V., Zhirnov, Vladimir A., Bormotov, Nikolay I., Skarnovich, Maksim O., Durymanov, Aleksander G., Molodtsova, Svetlana B., Filippova, Ekaterina I., Ovchinnikova, Alena S., Magerramova, Anastasia V., Ryzhikov, Alexander B., Maksyutov, Rinat A.
• Format: Journal Article
• Language: English
• Published: United States Hindawi 2021; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Animals; Antibiotic resistance; Antibiotics; Antiviral activity; Antiviral agents; Bacteria; Bifidobacterium bifidum; Bifidobacterium longum - genetics; Biomedical research; Care and treatment; Chlorocebus aethiops; Coronaviruses; COVID-19; COVID-19 - metabolism; COVID-19 - therapy; Dogs; Efflux; Gene sequencing; Genetic aspects; Genomes; Health aspects; High-Throughput Nucleotide Sequencing; Humans; Influenza; Influenza A; Influenza A Virus, H1N1 Subtype; Influenza, Human; Intestine; Lactobacillus - genetics; Lactobacillus fermentum; Madin Darby Canine Kidney Cells; Mass spectrometry; Mass spectroscopy; Microbiological research; Microorganisms; Pandemics; Plasmids; Probiotics; Probiotics - pharmacology; SARS-CoV-2 - metabolism; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Strains (organisms); Structure; Vero Cells; Viral diseases; Viruses; Whole genome sequencing; Russia; United States > US; Germany
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2021/6662027

Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.